In vivo pig models of venous thrombosis mimicking human disease
In vivo pig models of venous thrombosis mimicking human disease
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DOI：
10.1055/s-0037-1613440
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发表时间：
2003-02-01
影响因子：
6.7
通讯作者：
Drouet, L
中科院分区：
文献类型：
--
作者：
Kang, C;Bonneau, M;Drouet, L
静脉血栓形成的大多数动物模型涉及小型啮齿动物的急性血栓形成和高凝状态。为了更好地复制人类疾病，我们在猪中开发了两种模型，这是一种在大小以及血管和凝血反应性方面与人类相似的物种。一种模型涉及50%或80%狭窄的去内皮化，另一种模型涉及用Gore-TeX（TM）血管假体置换静脉段。在有和没有急性诱导高凝状态（凝血活酶输注）的情况下对两种模型进行了测试。未输注促凝血酶原激酶的血栓由多层血小板和纤维蛋白网状结构组成，与人体中常见的结构相似。输注促凝血酶原激酶后，血栓为包裹红细胞的均质纤维蛋白结构。使用80%狭窄模型获得的血栓形成的高发生率对于研究抗凝治疗是有用的，而使用50%狭窄模型获得的低发生率对于评价条件或治疗的促凝血作用是有用的。这些新模型进一步阐明了在与人类相似的条件下静脉血栓的发展，并可能被证明对研究抗凝和促凝作用有用。
Most animal models of venous thrombosis involve acute thrombosis with hypercoagulability in small rodents. To better replicate human disease, we developed two models in the pig, a species similar to humans in size and in vascular and coagulation reactivity. One model involves de-endothelialisation with 50% or 80% stenosis and the other replacement of a venous segment by a Gore-TeX(TM) vascular prosthesis. Both models were tested with and without acute induced hypercoagulability (thromboplastin infusion). Thrombi obtained without thromboplastin infusion were composed of a multilayered platelet and a fibrin meshwork structure similar to that usually found in humans. With thromboplastin infusion, the thrombi were homogeneous fibrin structures imprisoning red blood cells. The high incidence of thrombosis obtained with the 80% stenosis model would be useful for studying anticoagulant treatments, whereas the low incidence with 50% stenosis would be useful for evaluating procoagulant effects of conditions or treatments. These new models shed further light on the development of venous thrombi under conditions similar to those seen in humans and may prove useful for investigating anticoagulant and procoagulant effects.