Characterization of monocarboxylate transporter activity in hepatocellular carcinoma
Characterization of monocarboxylate transporter activity in hepatocellular carcinoma
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DOI：
10.3748/wjg.v20.i33.11780
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发表时间：
2014-09-07
影响因子：
4.3
通讯作者：
Baltazar, Fatima
中科院分区：
文献类型：
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作者：
Alves, Venancio A.;Pinheiro, Celine;Baltazar, Fatima
目的：评估肝硬化和原发性和转移性肝细胞癌（HCC）样本中缺氧相关标志物的免疫表达。方法：圣保罗大学医学院医院病理科共进行了5836例尸检，从2003年至2009年，188例原发性肝肿瘤。对80例HCC尸检标本进行单羧酸转运蛋白（MCT）-1，2和4，CD 147和葡萄糖转运蛋白-1（GLUT 1）的免疫组化反应性评估。使用IBM SPSS统计软件（版本19，IBM Company，阿尔蒙克，纽约州）储存和分析数据。使用Pearson卡方检验和Fisher精确检验（当n < 5时）检查所有比较的统计学显著性。结果：从非肿瘤性肝癌到原发性肝癌再到转移性肝癌，MCT 4的质膜表达和GLUT 1的总表达均逐渐增高。相反，从非肿瘤性到原发性肝癌和转移性肝癌，MCT 2的总体表达逐渐降低。MCT 1（总体和质膜表达）、MCT 2和CD 147质膜表达与无肝硬化相关，而CD 147质膜表达也与无HBV感染相关。MCT 2的总体表达与较低的肝脏重量、无转移和无腹腔播散相关。结论：MCT 4和GLUT 1在HCC的进展中起作用，而MCT 2在进展过程中丢失，预后较好。(C)2014百世登出版集团股份有限公司All rights reserved.
AIM: To assess the immunoexpression of hypoxia-related markers in samples from cirrhosis and primary and metastatic hepatocellular carcinoma (HCC).METHODS: From a total of 5836 autopsies performed at the Pathology Department - University of Sao Paulo School of Medicine Hospital - from 2003 to 2009, 188 presented primary liver tumors. Immunohistochemical reactivity for monocarboxylate transporters (MCTs)-1, 2 and 4, CD147 and glucose transporter-1 (GLUT1) was assessed in necropsies from 80 cases of HCC. Data were stored and analyzed using the IBM SPSS statistical software (version 19, IBM Company, Armonk, NY). All comparisons were examined for statistical significance using Pearson's chi(2) test and Fisher's exact test (when n < 5). The threshold for significant P values was established as P < 0.05.RESULTS: Plasma membrane expression of MCT4 and overall expression of GLUT1 showed progressively higher expression from non-neoplastic to primary HCC and to metastases. In contrast, overall expression of MCT2 was progressively decreased from non-neoplastic to primary HCC and to metastases. MCT1 (overall and plasma membrane expression), MCT2 and CD147 plasma membrane expression were associated with absence of cirrhosis, while plasma membrane expression of CD147 was also associated with absence of HBV infection. MCT2 overall expression was associated with lower liver weight, absence of metastasis and absence of abdominal dissemination. Additionally, MCT4 plasma membrane positivity was strongly associated with Ki-67 expression.CONCLUSION: MCT4 and GLUT1 appear to play a role in HCC progression, while MCT2 is lost during progression and associated with better prognosis. (C) 2014 Baishideng Publishing Group Inc. All rights reserved.