The human MHC-restricted cellular response to herpes simplex virus type 1 is mediated by CD4+, CD8- T cells and is restricted to the DR region of the MHC complex.
The human MHC-restricted cellular response to herpes simplex virus type 1 is mediated by CD4+, CD8- T cells and is restricted to the DR region of the MHC complex.
复制标题
对 1 型单纯疱疹病毒的人类 MHC 限制性细胞反应由 CD4+、CD8-T 细胞介导，并且仅限于 MHC 复合体的 DR 区域。
作者：
D. Schmid
研究了体外培养的人细胞毒性T细胞对1型单纯疱疹病毒(HSV-1)应答的性质。在含有MHC限制性活性的HSV-1刺激的5天培养中，存在两个表型不同的细胞群，它们能够在5h 51Cr释放试验中裂解HSV-1感染的B细胞系。第一组是典型的II类限制性T细胞的CD4+、CD8-、CD16-细胞，而另一组具有CD4-、CD8-、CD16+NK细胞表型。通过使用抗CD16+C抗体从大量培养物中去除NK细胞组分，通常会导致以抗原特异的、HLA-DR限制性的方式杀死的细胞群。在某些情况下，抗CD16预处理的培养物保留了不受MHC产品限制的杀伤群。在任何情况下，没有证据表明有任何细胞毒T细胞被限制在I类抗原上。前体频率的极限稀释分析表明，在血清阳性个体中，大约每4000到8000个细胞中就有1个是HSV-1特异性的。用极限稀释法将HLAI、HLAII相合的靶点与自体靶点进行比较，得到的结果与散体培养检测系统的结果完全一致。
The nature of the in vitro human cytotoxic T-cell responder population to HSV type 1 (HSV-1) was studied. In 5-day HSV-1-stimulated cultures that contained MHC-restricted activity, two phenotypically distinct populations of cells were present that were capable of lysing HSV-1-infected B cell lines in a 5-h 51Cr-release assay. The first was CD4+, CD8-, CD16- cell typical of class II-restricted T cells, whereas the other population bore a CD4-, CD8-, CD16+ NK-cell phenotype. Elimination of the NK cell fraction from bulk cultures by using anti-CD16 plus C frequently resulted in cell populations that killed in an Ag-specific, HLA-DR-restricted fashion. In some cases the anti-CD16-pretreated cultures retained a killing population that was unrestricted to MHC products. In no instance were any cytotoxic T cells that were restricted to class I Ag in evidence. Limiting dilution analysis of precursor frequency indicated that about 1 in 4000 to 1 in 8000 cells from peripheral blood are specific for HSV-1 in seropositive individuals. Comparisons of HLA class I-matched and HLA class II-matched targets with the autologous target by using limiting dilution analysis yielded results entirely consistent with those obtained in the bulk culture assay system.