Separation of synthetic oligonucleotides on columns of microparticulate silica coated with crosslinked polyethylene imine.
Separation of synthetic oligonucleotides on columns of microparticulate silica coated with crosslinked polyethylene imine.
复制标题
在涂有交联聚乙烯亚胺的微粒二氧化硅柱上分离合成寡核苷酸。
DOI：
10.1016/0003-2697(83)90225-7
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发表时间：
1983
影响因子：
2.9
通讯作者：
Weith,HL
中科院分区：
文献类型：
--
作者：
Lawson,TG;Regnier,FE;Weith,HL
介绍了一种快速有效的高效离子交换层析分离寡核苷酸的方法。在5-μm直径的多孔硅胶上吸附一层聚乙烯亚胺，然后用多官能团环氧乙烷进行交联，制备了色谱柱。这种弱的阴离子交换矩阵被发现在均聚或杂聚中分离单核苷酸和含有多达20个残基的寡核苷是有用的。小型分析柱(4.2×150 mm)的容量从分析分离时的不到1微克到制备模式下的几毫克不等。事实证明，这些色谱柱在评估前体片段的纯度、监测寡核苷酸的化学合成以及在合成后分离反应产物方面特别有用。
A rapid and effective method for separating oligonucleotides using high-performance ion-exchange chromatography is described. Columns were prepared by adsorbing a layer of polyethylene imine on 5-μm-diameter porous silica followed by crosslinking with a multifunctional oxirane. This weak anion-exchange matrix was found to be useful in the separation of mononucleotides and oligonucleosides containing up to 20 residues in either homo- or heteropolymers. Small analytical columns (4.2 × 150 mm) had a capacity ranging from less than a microgram in analytical separations to several milligrams in the preparative mode. The columns have proven to be especially useful in assessing the purity of precursor blocks, monitoring the chemical synthesis of oligonucleotides, and isolating reaction products after the synthesis.