Towards rectifying limitations on species delineation in dusky salamanders (Desmognathus: Plethodontidae): An ecoregion-drainage sampling grid reveals additional cryptic clades
Towards rectifying limitations on species delineation in dusky salamanders (Desmognathus: Plethodontidae): An ecoregion-drainage sampling grid reveals additional cryptic clades
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DOI：
10.11646/zootaxa.4734.1.1
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发表时间：
2020-02-17
期刊：
影响因子：
0.9
通讯作者：
Lamb, Trip
中科院分区：
文献类型：
--
作者：
Beamer, David A.;Lamb, Trip
暗色蝾螈（Desmognathus）是一个大的，物种丰富的家庭Plethodontidae的组，虽然他们的系统关系已被广泛解决，大多数研究集中在特定的物种复合体，因此只提供零碎的系统发育的角度对属。最近的工作揭示Desmognathus是更丰富的分支35个单系分支与22个公认的物种比以前想象的，结果，反过来，提供动力，在分支和地理区域内的额外调查工作，迄今为止稀疏采样。我们设想并实施了一个采样制度相结合的IV级生态区和独立的河流流域，以产生一个地理网格，全面恢复所有系谱专属分支。我们在美国东部的Desmognathus分布区采集了超过550个种群的样本，并为536个样本生成了线粒体DNA序列数据（mtDNA; 1，991 bp）。贝叶斯系统发育重建所产生的单倍型显示45个单系分支，其中11个从未被列入一个全面的系统发育重建，并在任何分子系统调查中没有代表另外三个。虽然一般的限制与mtDNA数据排除新的物种划分，我们配置文件的45个分支，并指定名称为10个新的分支（以下协议以前的分支命名）。我们还重新定义了几个物种复合体，并建立了新的非正式物种复合体。我们的数据集，其中包含几乎所有目前公认的物种和大多数同义词的拓扑样本，将提供一个强大的框架，为未来的努力，划定物种内Desmognathus。
Dusky salamanders (Desmognathus) constitute a large, species-rich group within the family Plethodontidae, and though their systematic relationships have been addressed extensively, most studies have centered on particular species complexes and therefore offer only piecemeal phylogenetic perspective on the genus. Recent work has revealed Desmognathus to be far more clade rich-35 reciprocally monophyletic clades versus 22 recognized species-than previously imagined, results that, in turn, provide impetus for additional survey effort within clades and across geographic areas thus far sparsely sampled. We conceived and implemented a sampling regime combining level IV ecoregions and independent river drainages to yield a geographic grid for comprehensive recovery of all genealogically exclusive clades. We sampled over 550 populations throughout the distribution of Desmognathus in the eastern United States of America and generated mitochondrial DNA sequence data (mtDNA; 1,991 bp) for 536 specimens. A Bayesian phylogenetic reconstruction of the resulting haplotypes revealed forty-five reciprocally monophyletic clades, eleven of which have never been included in a comprehensive phylogenetic reconstruction, and an additional three not represented in any molecular systematic survey. Although general limitations associated with mtDNA data preclude new species delineation, we profile each of the 45 clades and assign names to 10 new clades (following a protocol for previous clade nomenclature). We also redefine several species complexes and erect new informal species complexes. Our dataset, which contains topotypic samples for nearly every currently recognized species and most synonymies, will offer a robust framework for future efforts to delimit species within Desmognathus.