Nucleation and growth in one dimension. II. Application to DNA replication kinetics
Nucleation and growth in one dimension. II. Application to DNA replication kinetics
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DOI：
10.1103/physreve.71.011909
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发表时间：
2005-01-01
影响因子：
2.4
通讯作者：
Bechhoefer, J
中科院分区：
文献类型：
--
作者：
Jun, S;Bechhoefer, J
受最近的DNA复制实验的启发，我们将一维成核生长模型应用于DNA复制动力学，重点研究如何从数据中提取随时间变化的成核速率I(T)和生长速度v。我们讨论了一般性的实验问题：即空间不均匀性、测量噪声和有限尺寸效应。在评估了这些因素对I(T)和v的测量的影响后，我们给出了实验设计的指导方针。然后在DNA复制实验的背景下讨论这些想法。
Inspired by recent experiments on DNA replication, we apply a one-dimensional nucleation-and-growth model to DNA-replication kinetics, focusing on how to extract the time-dependent nucleation rate I(t) and growth speed v from data. We discuss generic experimental problems: namely, spatial inhomogeneity, measurement noise, and finite-size effects. After evaluating how each of these affects the measurements of I(t) and v, we give guidelines for the design of experiments. These ideas are then discussed in the context of the DNA-replication experiments.