Identification of amide protons of glutathione in MR spectra of tumour cells
Identification of amide protons of glutathione in MR spectra of tumour cells
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DOI：
10.1002/nbm.1280
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发表时间：
2008-11-01
影响因子：
2.9
通讯作者：
Viti, Vincenza
中科院分区：
文献类型：
--
作者：
Grande, Sveva;Luciani, Anna Maria;Viti, Vincenza
在四种肿瘤细胞系T98G、MCF-7、A172和HeLa的H-1-MR谱的低场区域观察到了可归因于酰胺质子的信号，并在以前的研究中用于测量细胞内的pH。这两个细胞系(T98G和MCF-7)的光谱信号更强，其特征是谷胱甘肽(GSH)浓度较高。通过与不同pH值下溶液中GSH的H-1-MR波谱比较，确定谱峰归属于GSH的半胱氨酸和甘氨酸残基的NHS。用丁硫氨酸亚磺胺处理细胞内GSH浓度的改变在GSH脂肪信号和NHS火种检查中的强度也有类似的降低。因此，这项任务得到确认。版权所有(C)2008 John Wiley&Sons，Ltd.
Signals attributable to amide protons and used in previous studies to measure intracellular pH were observed in the low-field region of the H-1-MR spectra of four tumour cell lines: T98G, MCF-7, A 172 and HeLa. The signals were more intense in the spectra of the two cell lines (T98G and MCF-7) characterised by higher concentrations of glutathione (GSH). After comparison with H-1-MR spectra of GSH in solution at different pH values, the peaks were attributed to NHs of the Cys and Gly residues of GSH. Modification of the intracellular concentration of GSH by treatment with buthionine sulfoximine produced comparable decreases in the intensity of aliphatic signals of GSH and NHS tinder examination. The assignment was therefore confirmed. Copyright (c) 2008 John Wiley & Sons, Ltd.