Insight into mitochondrial structure and function from electron tomography
Insight into mitochondrial structure and function from electron tomography
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DOI：
10.1016/s0005-2728(02)00278-5
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发表时间：
2002-09-10
影响因子：
4.3
通讯作者：
Perkins, GA
中科院分区：
文献类型：
--
作者：
Frey, TG;Renken, CW;Perkins, GA
近年来，电子断层扫描提供了详细的线粒体三维模型，重新定义了我们对线粒体结构的概念。该模型揭示了由两个组件组成的内膜，即与外膜紧密相连的内边界膜（IBM）和突出到基质隔室中的嵴膜。这两个组件通过尺寸相对均匀的管状结构（称为嵴连接处）连接。嵴连接尺寸和形状的分布是通过基于膜弯曲能量的热力学模型预测的，但蛋白质也可能在确定内膜的构象中发挥作用。细胞凋亡过程中线粒体的结构研究结果表明，细胞色素 c 被释放，而外膜没有发生可检测到的破坏，也没有线粒体基质的广泛膨胀，这表明形成了足够大的外膜孔，以允许全细胞色素 c 通过。还讨论了 IBM 和嵴膜之间内膜功能的可能划分。 (C) 2002 Elsevier Science B.V 保留所有权利。
In recent years, electron tomography has provided detailed three-dimensional models of mitochondria that have redefined our concept of mitochondrial structure. The models reveal an inner membrane consisting of two components, the inner boundary membrane (IBM) closely apposed to the outer membrane and the cristae membrane that projects into the matrix compartment. These two components are connected by tubular structures of relatively uniform size called crista junctions. The distribution of crista junction sizes and shapes is predicted by a thermodynamic model based upon the energy of membrane bending, but proteins likely also play a role in determining the conformation of the inner membrane. Results of structural studies of mitochondria during apoptosis demonstrate that cytochrome c is released without detectable disruption of the outer membrane or extensive swelling of the mitochondrial matrix, suggesting the formation of an outer membrane pore large enough to allow passage of holo-cytochrome c. The possible compartmentation of inner membrane function between the IBM and the cristae membrane is also discussed. (C) 2002 Elsevier Science B.V All rights reserved.