Nanoparticle effects on rat alveolar epithelial cell monolayer barrier properties.
Nanoparticle effects on rat alveolar epithelial cell monolayer barrier properties.
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DOI：
10.1016/j.tiv.2007.04.003
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发表时间：
2007-12
期刊：
影响因子：
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通讯作者：
N. Yacobi;H. Phuleria;L. Demaio;C. Liang;C. Peng;C. Sioutas;Z. Borok;Kwang-Jin Kim;E. Crandall
中科院分区：
文献类型：
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作者：
N. Yacobi;H. Phuleria;L. Demaio;C. Liang;C. Peng;C. Sioutas;Z. Borok;Kwang-Jin Kim;E. Crandall
据报道，吸入的纳米颗粒会对人体健康产生有害影响。在本研究中，我们研究了超细环境颗粒悬浮液（UAPS）、聚苯乙烯纳米颗粒（PNP；带正电和负电；20、100、120nm）、量子点（QD；带正电和负电；30nm）和单壁碳纳米管（SWCNT）对肺泡上皮细胞屏障性能的影响。在不同浓度的尖端纳米颗粒存在和不存在的情况下，测量了大鼠初级肺泡上皮单层的跨单层电阻（Rt）和等效短路电流（Ieq）。在一些实验中，测定了放射标记甘露醇或菊糖的根尖到基底侧的通量，并分析了暴露于UAPS或swcnts后乳酸脱氢酶（LDH）的释放。结果显示，暴露于UAPS后24小时内，rtie显著降低，但甘露醇和菊粉的通量没有变化。带正电荷的QD显著降低rt（随后恢复），而带负电荷的QD则没有。在swcnts暴露后（随后恢复），rt显着下降。另一方面，PNP暴露对Rtor Ieq没有影响。暴露于UAPS或swcnts后，LDH释放未见显著增加。这些数据表明，由于尖端纳米颗粒暴露导致的肺泡上皮屏障特性的破坏可能涉及细胞运输途径的改变，并取决于特定纳米颗粒的组成、形状和/或表面电荷。
Inhaled nanoparticles have been reported to contribute to deleterious effects on human health. In this study, we investigated the effects of ultrafine ambient particulate suspensions (UAPS), polystyrene nanoparticles (PNP; positively and negatively charged; 20, 100, 120nm), quantum dots (QD; positively and negatively charged; 30nm) and single-wall carbon nanotubes (SWCNT) on alveolar epithelial cell barrier properties. Transmonolayer resistance (Rt) and equivalent short-circuit current (Ieq) of primary rat alveolar epithelial monolayers were measured in the presence and absence of varying concentrations of apical nanoparticles. In some experiments, apical-to-basolateral fluxes of radiolabeled mannitol or inulin were determined with or without apical UAPS exposure and lactate dehydrogenase (LDH) release was analyzed after UAPS or SWCNT exposure. Results revealed that exposure to UAPS decreased Rtand Ieqsignificantly over 24h, although neither mannitol nor inulin fluxes changed. Positively charged QD decreased Rtsignificantly (with subsequent recovery), while negatively charged QD did not. Rtdecreased significantly after SWCNT exposure (with subsequent recovery). On the other hand, PNP exposure had no effects on Rtor Ieq. No significant increases in LDH release were observed after UAPS or SWCNT exposure. These data indicate that disruption of alveolar epithelial barrier properties due to apical nanoparticle exposure likely involves alteration of cellular transport pathways and is dependent on specific nanoparticle composition, shape and/or surface charge.