Non-apoptotic Cell Death Control of Neutrophil Extracellular Trap Formation.
Non-apoptotic Cell Death Control of Neutrophil Extracellular Trap Formation.
复制标题
中性粒细胞胞外陷阱形成的非凋亡细胞死亡控制。
DOI：
10.1007/978-1-0716-2449-4_16
复制
发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Croker,BenA
中科院分区：
文献类型：
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作者：
D'Cruz,AkshayA;Ericsson,Maria;Croker,BenA
中性粒细胞胞外陷阱（NET）是由中性粒细胞响应于感染和组织损伤而释放的染色质和杀微生物蛋白的网络。虽然传统上被视为中性粒细胞中的离散生化和细胞过程，但整合不同上游激活信号以控制NET形成的效应子途径（NETosis）定义不清。细胞死亡是中性粒细胞的一个常见的统一终点，现在描述了几种真正的非凋亡细胞死亡激动剂来启动NETosis。将这些新的遗传发现整合到我们现有的NETosis知识中，可能会揭示控制NET释放的各种细胞和生化过程以及由特定非凋亡细胞死亡触发的NET的特定抗微生物和炎症效应子功能。为了促进NETosis中调节细胞死亡途径的研究，我们提供了一个详细的方案，用于从小鼠骨髓和人血液中纯化中性粒细胞，通过流式细胞术分析NETs，并通过免疫金电子显微镜进行验证。未来的研究可能会更好地定义NETosis的细胞死亡特异性形式及其对炎症和自身免疫的影响。
Neutrophil extracellular traps (NETs) are networks of chromatin and microbicidal proteins released by neutrophils in response to infection and tissue damage. Although classically viewed as a discrete biochemical and cellular process in neutrophils, the effector pathways integrating diverse upstream activating signals to control the formation of NETs (NETosis) are poorly defined. Cell death is one such common unifying endpoint of neutrophils, with several bona fide non-apoptotic cell death agonists now described to initiate NETosis. Integrating these new genetic findings into our existing knowledge of NETosis will likely reveal varied cellular and biochemical processes controlling NET release and specific anti-microbial and inflammatory effector functions of NETs triggered by specific non-apoptotic cell death. To facilitate investigation of regulated cell death pathways in NETosis, we offer a detailed protocol for neutrophil purification from mouse bone marrow and human blood, analysis of NETs by flow cytometry, and validation by immunogold electron microscopy. Future studies may better define cell death-specific forms of NETosis and their influence on inflammation and autoimmunity.