Isolation and characterization of Vibrio vulnificus inhabiting the marine environment of the southwestern area of Taiwan
Isolation and characterization of Vibrio vulnificus inhabiting the marine environment of the southwestern area of Taiwan
复制标题
DOI：
10.1007/bf02255226
复制
发表时间：
1995-01-01
影响因子：
11
通讯作者：
Wan, Lei
中科院分区：
文献类型：
--
作者：
Hor, Lien-I.;Goo, Chi-Tai;Wan, Lei
创伤弧菌是一种海洋细菌，在台湾引起关注，因为它会引起伤口感染和败血症，每年的死亡率都很高。为了检测创伤弧菌，从云林、嘉义和台南的9个地点采集了13份海水或牡蛎样本。从11份样品中分离到77株创伤弧菌。在这些环境分离株中，72株(91%)为吲哚阳性，这是生物型1的特征。其余5株虽然吲哚阴性，但都是鸟氨酸脱羧酶和甘露醇阳性，这在生物型2菌株中从未报道过。根据商业鉴定系统获得的所有生化反应，这些吲哚阴性菌株似乎更像生物型1。在这些菌株中鉴定出57个核糖型，表明该物种之间存在巨大的遗传差异。在测试的30株环境分离株中，17株(56.7%)显示出与小鼠临床分离株相当的毒力，这意味着海洋环境中很高比例的创伤弧菌可能对人类具有致病性。
Vibrio vulnificus, a marine bacterium, is of concern in Taiwan because it causes wound infections and sepsis with a high mortality rate every year. To examine for V. vulnificus, 13 samples of seawater or oysters were collected from nine sites in Yunlin, Chiayi, and Tainan. Seventy-seven strains of V. vulnificus were isolated from 11 samples. Among these environmental isolates, 72 (91%) were indole-positive, a characteristic of biotype 1. The remaining five strains although indole-negative, a characteristic previously found exclusively in biotype 2 strains, were all ornithine decarboxylase- and mannitol-positive, which has never been reported for biotype 2 strains. Based on the overall biochemical reactions obtained using a commercial identification system, these indole-negative strains appeared to be more like biotype 1. Fifty-seven ribotypes were identified among these isolates, indicating the great genetic divergence in this species. Of the 30 environmental isolates tested, 17 (56.7%) exhibited virulence comparable to the clinical isolates in the mouse, implying that a high proportion of the V. vulnificus strains in the marine environments might be pathogenic to humans.