Distribution of resveratrol and stilbene synthase in young grape plants (Vitis vinifera L. cv. Cabernet Sauvignon) and the effect of UV-C on its accumulation
Distribution of resveratrol and stilbene synthase in young grape plants (Vitis vinifera L. cv. Cabernet Sauvignon) and the effect of UV-C on its accumulation
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白藜芦醇和二苯乙烯合酶在葡萄幼苗（Vitis vinifera L. cv. Cabernet Sauvignon）中的分布以及UV-C对其积累的影响
DOI：
10.1016/j.plaphy.2009.12.002
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发表时间：
2010-02-01
影响因子：
6.5
通讯作者：
Huang, Wei-Dong
中科院分区：
文献类型：
--
作者：
Wang, Wei;Tang, Ke;Huang, Wei-Dong
目前的研究表明，白藜芦醇主要积累在葡萄果实的果皮中，但关于白藜芦醇在葡萄幼株（葡萄属vinifera L cv. 2.3.1.95 Cabernet Sauvignon）。白藜芦醇在葡萄植株的茎、腋芽、茎尖、叶柄、根和叶中均能检测到，含量范围为0.2 ~ 16.5mg kg（-1）FW。其中，茎韧皮部白藜芦醇含量最高，叶片含量最低。有趣的是，STS mRNA和蛋白质的水平在葡萄叶片中最高。免疫组化分析显示STS在不同器官中的分布具有组织特异性，与蛋白质含量的结果相似。亚细胞定位显示，不同组织的细胞壁处理的代表STS的金色颗粒最多。UV-C辐射对葡萄叶片中的白藜芦醇和STS都有强烈的刺激作用，其响应模式相似。以上结果表明，白藜芦醇和STS在葡萄中的分布具有器官特异性和组织特异性。UV-C诱导的白藜芦醇积累受STS转录和翻译水平的调控。(C)2009年Elsevier Masson SAS。All rights reserved.
Current research indicated that the resveratrol was mainly accumulated in the skin of grape berry, however, little is yet known about the distribution of resveratrol, as well as the regulation mechanism at protein level and the localization of stilbene synthase (malonyl-CoA:4-coumaroyl-CoA malonyltransferase; EC 2.3.1.95; STS), a key enzyme of resveratrol biosynthesis, in young grape plants (Vitis vinifera L cv. Cabernet Sauvignon). Resveratrol, whose constitutive level ranged from 0.2 mg kg(-1) FW to 16.5 mg kg(-1) FW, could be detected in stem, axillary bud, shoot tip, petiole, root and leaf of grape plants. Among them, stem phloems presented the most abundant of resveratrol, and the leaves presented the lowest. Interestingly, the level of STS mRNA and protein were highest in grape leaves. And the analysis of immunohistochemical showed the tissue-specific distribution of STS in different organs, presenting the similar results compared with the amount of protein. And the subcellular localization revealed that the cell wall in different tissues processed the most golden particles representing STS. Subjecting to UV-C irradiation, resveratrol and STS were both intensely stimulated in grape leaves, with the similar response pattern. Results above indicated that distribution of resveratrol and STS in grape was organ-specific and tissue-specific. And the accumulation of resveratrol induced by UV-C was regulated by transcriptional and translational level of STS. (C) 2009 Elsevier Masson SAS. All rights reserved.