EFFECT OF ETHANOL TREATMENT ON INDEXES OF CUMULATIVE OXIDATIVE STRESS
EFFECT OF ETHANOL TREATMENT ON INDEXES OF CUMULATIVE OXIDATIVE STRESS
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DOI：
10.1016/0926-6917(94)90011-6
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发表时间：
1994-08-03
期刊：
影响因子：
--
通讯作者：
GUO, SX
中科院分区：
文献类型：
--
作者：
BONDY, SC;GUO, SX
在脑和肝脏中研究了乙醇暴露对与氧化应激相关的几个参数的影响。乙醛或乙醇的单次给药能够提高肝脏中活性氧的产生速率，但这种作用在大脑皮层中不明显。谷氨酰胺合成酶对自由基的失活特别敏感，在肝脏中发现了这种酶的累积氧化损伤的证据，在大脑皮层中发现的程度较低。在单次注射乙醛或乙醇后或更长时间给药后，这种酶在肝脏中被抑制。肝脏也比大脑皮层更容易受到促氧化剂作用的影响，这是通过急性给药任一溶剂后谷胱甘肽的抑制来判断的。酶抑制代表暂时性的氧化事件可能是一个更敏感的程序比直接测量活性氧物种的形成率，并可能发现在检测长期低水平的促氧化剂活性的特殊效用。
The effect of ethanol exposure upon several parameters relating to oxidative stress has been examined in brain and liver. A single administration of either acetaldehyde or ethanol was able to enhance rates of generation of reactive oxygen species in liver but this effect was not apparent in the cerebral cortex. Glutamine synthetase is especially sensitive to inactivation by free radicals and evidence for cumulative oxidative damage to this enzyme was found in liver and to a lesser extent in cerebral cortex. This enzyme was depressed in liver after both a single injection of acetaldehyde or ethanol, or after more extended dosing. The liver was also more susceptible than cerebral cortex, to pro-oxidant effects as judged by depression of glutathione after acute dosing with either solvent. Enzyme inhibition representing temporally summated oxidative events may be a more sensitive procedure than direct measurement of rates of formation of active oxygen species and may find especial utility in the detection of prolonged low level pro-oxidant activity.