Restriction polymorphism maps of Neurospora crassa: updates
Restriction polymorphism maps of Neurospora crassa: updates
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粗糙脉孢菌限制性多态性图谱：更新
DOI：
10.4148/1941-4765.1507
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
J. Grotelueschen
中科院分区：
文献类型：
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作者：
R. L. Metzenberg;J. Grotelueschen
当一个基因或来自生物体的未知DNA片段被克隆出来后，根据已知标记绘制其染色体起源位置通常是有用的。本作品采用知识共享署名-相同方式共享4.0许可协议。本文发表于真菌遗传学报告：http://newprairiepress.org/fgr/vol35/iss1/17 Metzenberg， R.L.和J. Grotelueschen粗神经孢子虫的限制性多型图谱：更新当一个基因或来自生物体的未知DNA片段被克隆后，根据已知标记绘制其染色体起源位置通常是有用的。这样做的方法和材料，以及一些关于标记分离的数据，已经发表（Metzenberg et al. 1984）。神经孢子病学报。31:35-39；同上，同上。学会科学。美国1985年。82:2067 - 2071;Metzenberg and Grotelueschen, 1987。真菌遗传学报，34(4):344 - 344。以下数据包括1987年文章中两个杂交的先前得分，并包含来自我们自己实验室和其他实验室的相同两个杂交的新数据。正如1987年的文章所指出的，第一个杂交的38个分离体来自有序的asci，它们提供的信息比第二个杂交的18个分离体提供的信息更多。然而，这两种杂交已经在许多实验室中使用，并提供了来自两者的数据。隔离评分的编码方式与之前相同：“M”或“0”分别表示类似于Mauriceville父级或类似于oak - ridge衍生父级的隔离评分：“-”表示评分未完成或由于技术原因而模棱两可；而分离物1中的(O)和分离物6中的(M)对于第二个杂交的所有条带都意味着它们不是后代而是杂交的亲本菌株，并且根据定义为0和M。在这些杂交中，基因或DNA片段的标记是混合的。如前所述，有些是明显的基因符号（如this -4），并在位点汇编中被索引（Perkins et al. 1982）。Microbiol。启46:426 - 570)。像1984年的那篇文章那样，那些简单的数字，如33、1或18，前面没有0，是5S rnas的位点。含有冒号（如12:8B）的基因座是用Vollmer-Yanofsky克隆文库（Vollmer and Yanofsky 1986）中相应的cosmid进行探针印迹鉴定的。Proc。国家的。国家科学。美国83:4869 - 4873)。H3H4是组蛋白H3 + H4 （wodt et al. 1983）。核酸学报。11:5347-5360)。conloci与分生有关（Berlin and Yanofsky 1985）。Molec。细胞。生物5:839 - 848;如上,849 - 855)。名称以LZ和DB开头的位点是未知功能的任意DNA片段，分别由Ludwika Zagorska和David Butler在我们的实验室研究。哈佛商学院是“本垒”，在金赛的实验室进行研究。cat-2 （Simmons et al. 1987）。真菌遗传学报，34:55-56)是一种编码过氧化氢酶的基因，它是通过蛋白质多态性而不是DNA多态性来评分的；值得注意的是，它在Ascus E中的分离表明发生了基因转换。最后，大量以一个或多个0开头的基因座是报告给我们的数据，但其作者希望基因座在发表或五年后（以先到者为准）保持身份不明和他们自己的匿名。即使没有鉴定，结果也丰富了图谱，并帮助其他人将他们的克隆定位到染色体上。如果您发现这些数据有用，请将您自己的任何结果（包括来自随机片段和克隆“错误”的结果）抄写在本文相应页面的副本上，并将其发送给RLM。你可以要求给它分配一个保密的号码，或者如果你愿意让基因和你自己的身份被识别出来，这将被完成。如果我们在这方面进行合作，我们可以希望看到这张地图变得更加密集和越来越有用。由NIH Grant GM 08995支持。威斯康星大学生理化学系，麦迪逊，威斯康星53706
When a gene or an unidentified fragment of DNA from an organism has been cloned, it is often useful to map its site of chromosomal origin with respect to known markers. Creative Commons License This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. This regular paper is available in Fungal Genetics Reports: http://newprairiepress.org/fgr/vol35/iss1/17 Metzenberg, R.L. and J. Grotelueschen Restriction polymorphism maps of Neurospora crassa: updates When a gene or an unidentified fragment of DNA from an organism has been cloned, it is often useful to map its site of chromosomal origin with respect to known markers. The methods and materials for doing this, and some data on segregation of markers, has been published (Metzenberg et al. 1984. Neurospora Newsl. 31:35-39; ibid. Proc. Natl. Acad. Sci. U.S. 1985. 82:2067-2071; Metzenberg and Grotelueschen, 1987. Fungal Genetics Newsl. 34:3944). The following data include the previous scorings of two crosses from the 1987 article and contains new data on the same two crosses from our own lab, and from others. As noted in the 1987 article, 38 segregants from the first cross were taken from ordered asci, and provide somewhat more information than can be obtained from the 18 segregants which represent random spores from the second cross. Both crosses, however, have been used in a number of laboratories, and data from both are presented. The scoring of segregants is coded in the same way as before: "M" or "0" indicate segregants that are like the Mauriceville parent or like the Oak-Ridge-derived parent, respectively: "-" indicates that the scoring was not done or was equivocal for technical reasons; and (O) in Isolate I and (M) in Isolate 6 for all lanes of the second cross means that these are not progeny but are the parental strains of the cross, and are 0 and M by definition. The notation for genes or DNA fragments mapped in these crosses is a mixed one. As before, some are obvious gene symbols (e.g. thi-4) and are indexed in the compendium of loci (Perkins et al. 1982. Microbiol. Rev. 46:426-570). Those with simple numbers like 33 or 1 or 18, unprefaced by zeros, are the loci of 5S rDNAs, as in the 1984 article. Those containing a colon (e.g. 12:8B) are loci identified by probing blots with the corresponding cosmid from the Vollmer-Yanofsky clonal library (Vollmer and Yanofsky 1986. Proc. Natl. Acad Sci. U.S. 83:4869-4873). H3H4 is histone H3 + H4 (Woudt et al. 1983. Nucleic Acids Res. 11:5347-5360). con loci are associated with conidiation (Berlin and Yanofsky 1985. Molec. Cell. Biol. 5:839-848; ibid. 849-855). Loci with names starting with LZ and DB are arbitrary DNA fragments of unknown function, studied in our laboratory by Ludwika Zagorska and David Butler, respectively. hbs is "homebase", studied in J. Kinsey's laboratory. cat-2 (Simmons et al. 1987. Fungal Genetics Newsl. 34:55-56) is a catalase-encoding gene scored by protein polymorphism rather than DNA polymorphism; note that its segregation in Ascus E suggests the occurrence of a gene conversion. Finally, the substantial number of loci whose numbers begin with one or more zeros are data that have been reported to us, but whose authors would like the loci to remain unidentified and themselves to be anonymous until publication or five years have elapsed, whichever is first. Even without identification, the results enrich the map and help others map their clones to a chromosome. If you have found these data useful please pass on the favor by pencilling any results of your own, including those from random fragments and from "mistakes" in cloning onto a copy of the appropriate page from this article and sending it to RLM. You may ask that a number which preserves confidentiality be assigned to it, or if you are willing for the gene and yourself to be identified, that will be done. If we cooperate on this, we can hope to see this map become more densely and increasingly useful. Supported by NIH Grant GM 08995. Department of Physiological Chemistry, University of Wisconsin, Madison, WI 53706