Enhanced electrical power generation using flame-oxidized stainless steel anode in microbial fuel cells and the anodic community structure.
Enhanced electrical power generation using flame-oxidized stainless steel anode in microbial fuel cells and the anodic community structure.
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DOI：
10.1186/s13068-016-0480-7
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发表时间：
2016
影响因子：
6.3
通讯作者：
Yokoyama H
中科院分区：
文献类型：
--
作者：
Yamashita T;Ishida M;Asakawa S;Kanamori H;Sasaki H;Ogino A;Katayose Y;Hatta T;Yokoyama H
碳基材料通常用作微生物燃料电池（MFC）中的阳极，而金属和金属氧化物基材料由于电输出低而不经常使用。不锈钢是一种低成本材料，具有高导电性和物理强度。在这项研究中，我们研究了发电使用火焰氧化（FO）不锈钢阳极（SSA）在单室空气阴极MFC。将FO-SSA性能与未处理的SSA和碳布阳极（CCA）（一种常见的碳质电极）的性能进行比较。利用16 S rRNA基因V4区的高通量测序分析阳极群落结构的差异。火焰氧化SSA产生的凸起的节点状网站，主要由赤铁矿（Fe 2 O3），在表面上，通过X-射线衍射光谱法测定。火焰氧化提高了MFC的最大功率密度（1063 mW/m2），比未处理的SSA和CCA分别提高了184%和24%。在恒电位测试条件下，FO-SSA的电流产生分别比SSA和CCA高8.75和2.71倍。在FO-SSA上形成的生物膜（8.8- 9.2%）中检测到的来自地芽孢杆菌属的细菌的频率显著高于在SSA和CCA上形成的生物膜（0.7- 1.4%）。在FO-SSA生物膜中，与金属还原地芽孢杆菌密切相关的细菌物种（基因序列中的同源性> 99%）在地芽孢杆菌属中占优势（93- 96%），而与G.在SSA和CCA生物膜中，阳极氧化物含量丰富（> 55%）。这是首次在MFC中使用FO-SSA发电的演示。SSA的火焰氧化提高了MFC中的发电量，其高于普通碳质电极CCA。FO-SSA不仅价格便宜，而且可以使用简单的方法制备。据我们所知，这项研究首次揭示了生物膜中的主要Geodynamic物种取决于阳极材料。FO-SSA的高性能可能是由于与G.生物膜中的金属还原菌。本文的在线版本（doi：10.1186/s13068-016-0480-7）包含补充材料，可供授权用户使用。
Carbon-based materials are commonly used as anodes in microbial fuel cells (MFCs), whereas metal and metal-oxide-based materials are not used frequently because of low electrical output. Stainless steel is a low-cost material with high conductivity and physical strength. In this study, we investigated the power generation using flame-oxidized (FO) stainless steel anodes (SSAs) in single-chambered air-cathode MFCs. The FO-SSA performance was compared to the performance of untreated SSA and carbon cloth anode (CCA), a common carbonaceous electrode. The difference in the anodic community structures was analyzed using high-throughput sequencing of the V4 region in 16S rRNA gene. Flame oxidation of SSA produced raised node-like sites, predominantly consisting of hematite (Fe2O3), on the surface, as determined by X-ray diffraction spectroscopy. The flame oxidation enhanced the maximum power density (1063 mW/m2) in MFCs, which was 184 and 24 % higher than those for untreated SSA and CCA, respectively. The FO-SSA exhibited 8.75 and 2.71 times higher current production than SSA and CCA, respectively, under potentiostatic testing conditions. Bacteria from the genus Geobacter were detected at a remarkably higher frequency in the biofilm formed on the FO-SSA (8.8–9.2 %) than in the biofilms formed on the SSA and CCA (0.7–1.4 %). Bacterial species closely related to Geobacter metallireducens (>99 % identity in the gene sequence) were predominant (93–96 %) among the genus Geobacter in the FO-SSA biofilm, whereas bacteria with a 100 % identity to G. anodireducens were abundant (>55 %) in the SSA and CCA biofilms. This is the first demonstration of power generation using an FO-SSA in MFCs. Flame oxidation of the SSA enhances electricity production in MFCs, which is higher than that with the common carbonaceous electrode, CCA. The FO-SSA is not only inexpensive but also can be prepared using a simple method. To our knowledge, this study reveals, for the first time, that the predominant Geobacter species in the biofilm depends on the anode material. The high performance of the FO-SSA could result from the particularly high population of bacteria closely related to G. metallireducens in the biofilm. The online version of this article (doi:10.1186/s13068-016-0480-7) contains supplementary material, which is available to authorized users.