Development of rat antigen-presenting cells from pluripotent ecto-mesenchymal stem cells in vitro and in vivo.
Development of rat antigen-presenting cells from pluripotent ecto-mesenchymal stem cells in vitro and in vivo.
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DOI：
10.1016/j.molimm.2008.05.019
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发表时间：
2008-08
影响因子：
3.6
通讯作者：
Shijie Hu;Xue-Yan Shen;Rong Zhang;Yong-jie Zhang;Rui Zhang;Wei Zhang;Z. Deng;Yunxin Cao;Zeyuan Zhou;Jinwu Chen;Guanqun Ge;Kun Xuan;Xiang Zhang;Yan Jin
中科院分区：
文献类型：
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作者：
Shijie Hu;Xue-Yan Shen;Rong Zhang;Yong-jie Zhang;Rui Zhang;Wei Zhang;Z. Deng;Yunxin Cao;Zeyuan Zhou;Jinwu Chen;Guanqun Ge;Kun Xuan;Xiang Zhang;Yan Jin
树突状细胞（DC）是捕获并呈递抗原给T细胞的特化细胞。最近的进展已在了解其起源，异质性，并诱导其迁移和成熟的信号驻留小胶质细胞是抗原呈递细胞（APC）参与刺激或激活CNS靶向T细胞。如离体证明的那样，从小胶质细胞产生DC可能支持GM-CSF驱动的脑DC从局部可能的小胶质祖细胞分化。在这里，我们报告建立长期培养的大鼠外间充质干细胞（EMSC）使用特定的补充培养基诱导。这些EMSC共享一些形态学特征和经典DC的同种异体刺激能力，并且当使用大鼠胶质瘤模型移植到脑中时，在皮质内存活，并且在形态学和表型上与小胶质细胞相似超过7天。我们的研究结果有关的发展和分化的小胶质细胞祖细胞支持的观点，即小胶质细胞来源于产前中胚层祖细胞，是不同于单核细胞。
Dendritic cells (DC) are specialized cells that capture and present antigen to T cells. Recent advances have been made in understanding their origin, heterogeneity, and the signals that induce their migration and maturation resident microglia are antigen-presenting cells (APC) involved in stimulation or reactivation of CNS-targeted T cells. Generation of DC from microglia, as demonstrated ex vivo, may support GM-CSF-driven differentiation of brain DC from local, likely, microglial progenitors. Here, we report the establishment of long-term cultures of rat ecto-mesenchymal stem cells (EMSCs) using specific supplemented media for induction. These EMSCs share some morphological characteristics and the allostimulatory capacity of classical DCs, and when transplanted into the brain using a rat glioma model survive within the cortex, and are morphologically and phenotypically similar to microglia over 7 days. Our findings related to the development and differentiation of microglial progenitors support the view that microglia are derived prenatally from mesodermal progenitors that are distinct from monocytes.