DNA-damage induction by eight metal compounds in TK6 human lymphoblastoid cells: Results obtained with the alkaline Comet assay
DNA-damage induction by eight metal compounds in TK6 human lymphoblastoid cells: Results obtained with the alkaline Comet assay
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DOI：
10.1016/j.mrgentox.2008.04.005
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发表时间：
2008-06-30
影响因子：
1.9
通讯作者：
Marcos, Ricardo
中科院分区：
文献类型：
--
作者：
Guillamet, Emma;Creus, Amadeu;Marcos, Ricardo
金属化合物寿命长，可以与不同的大分子反应，产生广泛的生物效应，包括DNA损伤。由于它们的反应性与其化学结构有关，因此获得同一金属中一种以上化合物的信息非常重要。在这项研究中，DNA损伤的潜力，两种汞化合物（氯化汞和氯化甲基汞），两种镍化合物（氯化镍和六氟镍酸钾），两种钯化合物（四氯钯酸铵和六氯钯酸铵），和两种碲化合物（亚碲酸钠和碲酸钠）在人类淋巴母细胞TK6细胞中使用的彗星试验的碱性版本进行了评估。随着计算机图像分析系统收集彗星数据的使用越来越多，用于量化DNA损伤的度量标准是橄榄尾矩。治疗持续3小时，测试的浓度范围是不同的每种金属化合物，这取决于其毒性。两种汞剂都对TK6细胞产生DNA损伤，氯化汞比氯化甲基汞产生的DNA损伤大得多。在这两种镍化合物中，只有氯化镍（Ni（II）化合物）诱导DNA断裂。类似地，在两种钯化合物中，仅Pd（II）化合物（四氯钯酸铵）在测定中为阳性。亚碲酸钠明显呈阳性，产生浓度相关的DNA损伤增加，而碲酸钠则呈阴性反应。总之，当用彗星试验测量时，所选金属化合物在人TK6细胞中诱导DNA损伤的能力取决于化学形式，并且通常，含有较低价态金属的化合物显示出更大的DNA损伤能力。(c)2008 Elsevier B.V.保留所有权利。
Metal compounds are long-lived and can react with different macromolecules, producing a wide range of biological effects, including DNA damage. Since their reactivity is associated with their chemical structure, it is important to obtain information on more than one compound from the same metal. In this study, the DNA-damaging potential of two mercury compounds (mercury chloride and methyl mercury chloride), two nickel compounds (nickel chloride and potassium hexafluoronickelate), two palladium compounds (ammonium tetrachloropalladate and ammonium hexachloropalladate), and two tellurium compounds (sodium tellurite and sodium tellurate) was evaluated in human lymphoblastoid TK6 cells by use of the alkaline version of the Comet assay. As the use of computerized image-analysis systems to collect comet data has increased, the metric used for quantifying DNA damage was the Olive tail moment. Treatments lasted for 3 h and the range of concentrations tested was different for each metal compound, depending on its toxicity. Both mercury agents produced DNA damage in TK6 cells, with mercury chloride producing considerably more DNA damage than methyl mercury chloride. Of the two nickel compounds, only nickel chloride (a Ni(II) compound) induced DNA breaks. Similarly, of the two palladium compounds, only the Pd(II) compound (ammonium tetrachloropalladate) was positive in the assay. Sodium tellurite was clearly positive, producing concentration-related increases in DNA damage, while sodium tellurate gave a negative response. In conclusion, the ability of inducing DNA damage by the selected metal compounds in human TK6 cells, when measured with the Comet assay, was dependent on the chemical form and, in general, compounds containing the metal in the lower valence state displayed the greater DNA-damaging ability. (c) 2008 Elsevier B.V. All rights reserved.