Relation between stage, grade, proliferation, and expression of p53 and CD44 in adenomas and carcinomas of the colorectum.
Relation between stage, grade, proliferation, and expression of p53 and CD44 in adenomas and carcinomas of the colorectum.
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结直肠腺瘤和癌中 p53 和 CD44 的分期、分级、增殖和表达之间的关系。
DOI：
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发表时间：
1995
影响因子：
3.4
通讯作者：
M G Cook
中科院分区：
文献类型：
--
作者：
P A Jackson;M A Green;A. Pouli;R. Hubbard;C G Marks;M G Cook
目的：研究p53、CD 44和MIB-1在结直肠腺癌中表达的变化及其相互关系，并确定这些变化是否在腺瘤-癌序列中是进行性的。方法：采用免疫组化法检测68例大肠癌和32例大肠腺瘤中p53蛋白、CD 44粘附分子和MIB-1增殖抗原的表达。将染色特征与腺瘤的异型增生程度、癌的分化程度和Dukes分期进行比较。采用斯皮尔曼等级相关和独立t检验对结果进行分析和评估。结果：p53蛋白在SOM腺瘤中表达，并与异型增生程度相关。癌的表达明显高于腺瘤，且与Dukes分期有明显相关性。CD 44染色在腺瘤中最大，在癌中减少，在转移癌中最小。在腺瘤-癌-转移癌序列中，p53和CD 44表达呈负相关。MIB-1在癌中表达最高，但与p53或CD 44表达无关。结论：腺瘤和癌中p53、CD 44和MIB-1表达呈进行性变化。这些测试的组合可能被证明是有用的，在评估哪些腺瘤患者是在最大的风险进展为癌症。
AIMS--To investigate the changes in and relations among p53, CD44 and MIB-1 expression in adenocarcinomas of the colorectum and to determine whether these changes are progressive across the adenoma-carcinoma sequence. METHODS--Expression of p53 protein, CD44 adhesion molecule and MIB-1 proliferation antigen was detected using immunohistochemistry in 68 colorectal carcinomas and 32 colorectal adenoma. The staining characteristics were compared with degree of dysplasia in adenomas, and differentiation and Dukes' stage in carcinomas. Results were analysed and assessed using Spearman's rank correlation and independent t tests. RESULTS--p53 staining was present in som adenomas and correlated with the degree of dysplasia. There was significantly more staining in carcinomas than adenomas and significant correlation between staining and Dukes' stage. CD44 staining was maximal in adenomas, diminished in carcinomas and was minimal in metastasising carcinomas. There was inverse correlation between p53 and CD44 expression across the adenoma-carcinoma-metastasising carcinoma sequence. MIB-1 expression was highest in carcinomas but did not correlate with either p53 or CD44 expression. CONCLUSIONS--There are progressive changes in p53, CD44 and MIB-1 expression in adenomas and carcinomas. A combination of these tests may prove useful in assessing which patients with adenomas are at greatest risk of progressing to carcinoma.
影响因子：
29.4
作者：
M. O'brien;S. Winawer;A. Zauber;L. Gottlieb;S. Sternberg;B. Diaz;G. Dickersin;Stephen Ewing;Stephen Geller;D. Kasimian;R. Komorowski;A. Szporn
通讯作者：
M. O'brien;S. Winawer;A. Zauber;L. Gottlieb;S. Sternberg;B. Diaz;G. Dickersin;Stephen Ewing;Stephen Geller;D. Kasimian;R. Komorowski;A. Szporn