Nerve growth factor and the peptidergic sensory neurons
Nerve growth factor and the peptidergic sensory neurons
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神经生长因子和肽能感觉神经元
DOI：
10.1016/0165-6147(84)90453-x
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发表时间：
1984
影响因子：
13.8
通讯作者：
U. Otten
中科院分区：
文献类型：
--
作者：
U. Otten
神经生长因子 (NG F~ 是外周交感神经系统正常发育和功能维持所必需的。给新生儿施用 mui-NGF 抗体会导致其交感神经节不可逆破坏（免疫交感神经切除术）。但 NGF 的轴突转运并不限于肾上腺素能神经元。本报告总结了最近的研究结果，表明内源性 NGF 对于神经元的发育和功能都至关重要。肽能血清）'神经元。 NGF 对背根神经节神经元的特异性作用似乎受到糖皮质激素的调节。这种现象与糖皮质激素对交感神经节、肾上腺髓质和大鼠嗜铬细胞瘤细胞中 NG F 诱导作用的特征作用有关。神经生长因子 (NGF) 最初是在研究某些小鼠纤维肉瘤的生长促进作用的过程中发现的。将小鼠肉瘤组织移植到鸡胚胎的体壁中会导致感觉神经节和交感神经节的大小增加。 Levi Montalcini和Hamburger (1951)表明，这种肿瘤由感觉神经和交感神经纤维密集支配，其作用是由于肉瘤组织产生的体液因子所致。开发了一种特定的生物测定法，通过确定其从外植的鸡胚感觉神经节产生神经纤维生长的能力，以半定量的方式测量 NGF。这种生物测定法可以确定多种组织和体液中 NGF 的存在，包括成年雄性小鼠的下颌下腺、蛇毒、豚鼠、兔子、公牛和绵羊的前列腺，以及公牛和其他物种的精浆。成年雄性小鼠的颌下腺被发现含有非常高浓度的 NGF，并且它仍然是分离这种生长因子的主要来源。从小鼠颌下腺中提取的蛋白质是一种称为 7S NGF 的多组分蛋白质，它包含具有生物活性的 13 亚基和其他两个亚基，et 和 ~，它们不刺激神经生长 2。NGF 是周围交感神经正常发育和维持功能所必需的。
Nerve growth factor (NG F~ is required for nornml developmon and maintenance of function of the peripheral sympathetic nervolts system. Administration of mui-NGF antibody to neonates leads to irreversible destruction of their sympathetic ganglia (immunosympathectomy). But axonal transport of NGF is not limited to adrenergic neurons. This report presents a summary of recent findings showing that endogenom NGF is essential for both development mtd fimction of peptidergic semor)'neurons. Specific NGF action on dorsal root ganglia neurons appears to be modulated by glucocorticoids. This phenomenon is atudogous to the characteristic action of glucocorticoids on NG F induced effects in sympathetic ganglia, in adrenal medulhtrv and in rat pheochromocytoma cells.Nerve growth factor (NGF) was originally discovered in the course of investigations on the growth-promoting effects of certain mouse fibrosarcomas. Mouse sarcoma tissue grafted into the body wall of chick embryos produced an increase in the size of sensory and sympathetic ganglia. Levi Montalcini and Hamburger (1951) showed that this tumor was densely innervated by sensory and sympathetic nerve fibers and that the effects were due to a humoral factor produced by the sarcoma tissue. A specific bioassay was developed which measured NGF in a semiquantitative fashion by determining" its ability to produce outgrowth of nerve fibers from explanted chick embryo sensory ganglia. This bioassay made it possible to determine the presence of NGF in a variety of tissues and body fluids including the submandibular gland of the adult male mouse, snake venoms, prostate glands of the guinea pig, rabbit, bull and sheep, and in seminal plasma of the bull and other species. The submandibular gland of adult male mouse was found to contain very high concentrations of NGF and it still represents the major source for isolating this growth factor. The protein extracted from mouse submandibular glands is a multicomponent protein known as 7S NGF. It contains the biologically active 13-subunit and two other subunits, et and~, which do not stimulate nerve growth 2. NGF is required for the normal development and maintenance of function of the peripheral sympathetic