Non-invasive detection of apoptosis using magnetic resonance imaging and a targeted contrast agent
Non-invasive detection of apoptosis using magnetic resonance imaging and a targeted contrast agent
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DOI：
10.1038/nm1101-1241
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发表时间：
2001-11-01
期刊：
影响因子：
82.9
通讯作者：
Brindle, KM
中科院分区：
文献类型：
--
作者：
Zhao, M;Beauregard, DA;Brindle, KM
的C-2结构域的突触结合蛋白I，结合到细胞膜中的阴离子磷脂，被证明结合到细胞凋亡细胞的质膜通过流式细胞术和共聚焦显微镜。将蛋白质与超顺磁性氧化铁纳米颗粒结合，可以使用磁共振成像检测这种结合。凋亡细胞的检测，使用这种新的造影剂，证明了在体外，与孤立的凋亡肿瘤细胞，并在体内，在用化疗药物治疗的肿瘤。
The C-2 domain of synaptotagmin I, which binds to anionic phospholipids in cell membranes, was shown to bind to the plasma membrane of apoptotic cells by both flow cytometry and confocal microscopy. Conjugation of the protein to superparamagnetic iron oxide nanoparticles allowed detection of this binding using magnetic resonance imaging. Detection of apoptotic cells, using this novel contrast agent, was demonstrated both in vitro, with isolated apoptotic tumor cells, and in vivo, in a tumor treated with chemotherapeutic drugs.