Kaempferol reduces obesity, prevents intestinal inflammation, and modulates gut microbiota in high-fat diet mice
Kaempferol reduces obesity, prevents intestinal inflammation, and modulates gut microbiota in high-fat diet mice
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山奈酚可减少高脂肪饮食小鼠的肥胖、预防肠道炎症并调节肠道微生物群
DOI：
10.1016/j.jnutbio.2021.108840
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发表时间：
2021-09-09
影响因子：
5.6
通讯作者：
Zhang, Bingkun
中科院分区：
文献类型：
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作者：
Bian, Yifei;Lei, Jiaqi;Zhang, Bingkun
山奈酚是一种在多种膳食来源中被发现的类黄酮，据报道具有抗肥胖特性；然而，其潜在机制了解甚少。慢性、低度肠道炎症和菌群失调被认为是肥胖相关疾病的潜在因素以及新的治疗方法。本研究旨在探讨山奈酚实验性治疗对肥胖动物模型肠道炎症和肠道微生物平衡的益处。给C57BL/6J小鼠喂食高脂肪饮食（HFD）16周，在此期间山奈酚的补充作为一个变量。显然，HFD诱导了肥胖、脂肪堆积、葡萄糖不耐受和脂肪炎症，这是主要发现的代谢综合征。所有这些代谢紊乱都可以通过补充山奈酚得到缓解。此外，在HFD诱导的小鼠中还发现肠道通透性增加、免疫细胞（巨噬细胞、树突状细胞和中性粒细胞）浸润以及炎症细胞因子（肿瘤坏死因子 -α、白细胞介素 -1β、白细胞介素 -6、单核细胞趋化蛋白 -1）过度表达。山奈酚补充通过减少TLR4/NF -κB通路的激活，改善了肠道屏障完整性并抑制了肠道炎症。此外，通过测序对盲肠微生物群进行的特征分析表明，山奈酚补充能够抵消与肥胖相关的菌群失调。我们的研究描绘了山奈酚抗肥胖作用的多种潜在作用机制，并为支持将山奈酚开发为治疗肥胖的膳食补充剂提供了科学依据。（C）2021爱思唯尔公司。保留所有权利。
Kaempferol, a flavonoid identified in a wide variety of dietary sources, has been reported to possess anti-obesity properties; however, its underlying mechanism was poorly understood. Chronic, low-grade gut inflammation and dysbacteria are proposed as underlying factors as well as novel treatment approaches for obesity-associated pathologies. This present study aims to investigate the benefits of experimental treatment with kaempferol on intestinal inflammation and gut microbial balance in animal model of obesity. High fat diet (HFD) was applied to C57BL/6J mice for 16 weeks, during which the supplement of kaempferol served as a variable. Clearly, HFD induced obesity, fat accumulation, glucose intolerance and adipose inflammation, the metabolic syndrome of which was the main finding. All these metabolic disorders can be alleviated through kaempferol supplementation. In addition, increased intestinal permeability, infiltration of immunocytes (macrophage, dendritic cells and neutrophils) and overexpression of inflammatory cytokines (tumor necrosis factor-alpha, interleukin-1beta, interleukin-6, monocyte chemoattractant protein-1) were also found in the HFD-induced mice. Kaempferol supplementation improved intestinal barrier integrity and inhibited gut inflammation, by reducing the activation of TLR4/NF-kappa B pathway. Furthermore, the characterization of the cecal microbiota by sequencing showed that kaempferol supplementation was able to counteract the dysbiosis associated to obesity. Our study delineated the multiple mechanism of action underlying the anti-obesity effect of kaempferol, and provide scientific evidence to support the development of kaempferol as a dietary supplement for obesity treatment. (C) 2021 Elsevier Inc. All rights reserved.