Coordinating cell fate and morphogenesis in Drosophila renal tubules
Coordinating cell fate and morphogenesis in Drosophila renal tubules
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DOI：
10.1098/rstb.2000.0628
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发表时间：
2000-07-29
期刊：
影响因子：
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通讯作者：
Skaer, H
中科院分区：
文献类型：
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作者：
Ainsworth, C;Wan, S;Skaer, H
以果蝇的肾小管为例，我们探讨了细胞规格如何导致组织结构产生的形态发生运动。以两个发育阶段为例，我们首先表明，小管细胞是通过后肠内胚层和外胚层间的信号传导来分配的。Wnt通路的激活影响外胚层的形成，导致小管基因在一部分细胞中表达，并从后肠外翻形成小管原基。我们认为，早期基因表达指导这些形态发生运动，但不是小管分化的完整程序。在第二个例子中，我们展示了有丝分裂尖端细胞谱系在每个小管中的分配不仅是细胞分裂的正常模式所必需的，也是成熟小管的定型三维排列所必需的。对尖端细胞谱系被错误指定的突变体的分析表明，小管在体腔中导航需要尖端细胞祖细胞的两个子细胞，因此远端尖端位于其特征位置。我们发现Rac的调节因子Myoblast city在这第二次形态发生过程中是必不可少的。
Using the renal tubules of Drosophila as an example, we explore how cell specification leads to the morphogenetic movements that underlie the generation of tissue architecture. Taking two stages of development, we show first that the tubule cells are allocated by signalling between the endodermal and ectodermal compartments of the posterior gut. Activation of the Wnt pathway patterns the ectodermal anlage resulting in the expression of tubule genes in a subset of cells and their eversion from the hindgut to form the tubule primordia. We argue that early gene expression directs these morphogenetic movements but not the complete programme of tubule differentiation. In the second example we show that the allocation of the mitogenic tip cell lineage in each tubule is required not only for the normal pattern of cell division but also for the stereotyped three-dimensional arrangement of the mature tubules. Analysis of mutants in which the tip cell lineage is misspecified reveals that both daughters of the tip cell progenitor are required for the tubules to navigate through the body cavity, so that the distal tips locate in their characteristic positions. We show that the regulator of Rac, Myoblast city, is essential for this second morphogenetic process.