IgG subclass-associated affinity differences of specific antibodies in humans.
IgG subclass-associated affinity differences of specific antibodies in humans.
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人类特异性抗体的 IgG 亚类相关亲和力差异。
DOI：
10.4049/jimmunol.140.11.3875
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发表时间：
1988
影响因子：
4.4
通讯作者：
A. Carbonara
中科院分区：
文献类型：
--
作者：
M. Persson;S. E. Brown;M. Steward;L. Hammarström;C. I. Smith;C. Howard;M. Wahl;B. Rynnel;G. Lefranc;A. Carbonara
人IgG亚类在其生物学功能上不同，并且仅限于作为针对某些Ag的特异性抗体。这种限制的基础是未知的，但为了进一步表征它，我们获得了血清制剂含有一个单一的亚类通过使用亚类特异性单克隆抗体在亲和层析。随后，我们测定了四种IgG亚类中抗体对gp 30/p25（一种B型肝炎表面Ag（HBsAg）复合物）的亲和力，对代表HBsAg残基139至147的9个氨基酸的环状肽的亲和力，和代表前-乙型肝炎B病毒残基126至140的肽，S2区和IgG 1和IgG 2抗体对纯化的肺炎球菌多糖3型的相对亲合力。对HBsAg的亲和力在来自接种疫苗和来自自然感染个体的血清中均显示出明显的亲和力降低模式，其顺序为IgG 1大于IgG 2大于IgG 3大于IgG 4。抗体对多糖Ag的相对亲合力具有相反的模式，IgG 2大于IgG 1。在有或没有IG H链基因缺失的个体中，抗HBsAg应答仅限于一个亚类，亲和力与在来自另外具有其他亚类的高或低亲和力抗体的个体的血清中观察到的相同亚类的亲和力相似。
Human IgG subclasses differ in their biologic functions and are restricted as specific antibodies to certain Ag. The basis for this restriction is unknown but in order to characterize it further, we obtained serum preparations containing one single subclass by using subclass-specific mAb in affinity chromatography. Subsequently we determined the affinity of antibodies in the four IgG subclasses for gp30/p25, a hepatitis B surface Ag (HBsAg) complex, for a nine-amino acid cyclical peptide representing residues 139 to 147 of HBsAg, and for a peptide representing residues 126 to 140 of the hepatitis B virus pre-S2 region and the relative avidity of IgG1 and IgG2 antibodies for purified pneumococcal polysaccharide type 3. Affinities to the HBsAg showed a clear pattern of decreasing affinity in the order IgG1 greater than IgG2 greater than IgG3 greater than IgG4 both in sera from vaccinated and from naturally infected individuals. The relative avidities of antibodies to the polysaccharide Ag had a reverse pattern, IgG2 greater than IgG1. In individuals with or without Ig H chain gene deletions where the anti-HBsAg response was restricted to one subclass, the affinity was similar to that observed for the same subclass in sera from individuals who in addition possessed high or low affinity antibodies of other subclasses.