Therapeutic efficacy of cyclosporin A against spinal cord injury in rats with hyperglycemia.
Therapeutic efficacy of cyclosporin A against spinal cord injury in rats with hyperglycemia.
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DOI：
10.3892/mmr.2018.8422
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发表时间：
2018-03
影响因子：
3.4
通讯作者：
Jin QH
中科院分区：
文献类型：
--
作者：
Chen ZR;Ma Y;Guo HH;Lu ZD;Jin QH
本研究旨在探讨环孢菌素A（CsA）对高血糖大鼠脊髓损伤（SCI）的治疗作用，寻找一种治疗高血糖SCI的新方法。雌性SD大鼠随机分为假手术组、SCI组、SCI+高血糖组和SCI+高血糖+CsA组。建立链脲佐菌素诱导的高血糖SD大鼠和大鼠急性脊髓损伤模型。采用Basso、Beattie、Bresnahan评分法和斜板试验评价大鼠的神经功能。流式细胞术检测脊髓细胞凋亡率。采用酶联免疫吸附试验（ELISA）和蛋白质印迹法（Western blot）检测各组血清白细胞介素（IL）-10、肿瘤坏死因子（TNF）-α、亲环素D（Cyp-D）和凋亡诱导因子（AIF）水平。结果表明，CsA对高血糖大鼠的神经功能有明显的改善作用。CsA可明显减少高血糖引起的脊髓凋亡细胞数。CsA可显著降低脊髓损伤大鼠脊髓组织中IL-10、TNF-α、Cyp-D和AIF的表达水平。总之，本研究揭示了CsA通过抑制脊髓细胞凋亡在治疗高血糖的SCI中的重要作用。
The present study aimed to explore the therapeutic effects of cyclosporin A (CsA) on spinal cord injury (SCI) in rats with hyperglycemia and to identify a novel potential method to treat SCI in the presence of hyperglycemia. Female Sprague-Dawley (SD) rats were randomly allocated into four groups: Sham, SCI, SCI+hyperglycemia and SCI+hyperglycemia+CsA groups. Streptozotocin-induced hyperglycemic SD rats and a weight-drop contusion SCI model were established. The Basso, Beattie, Bresnahan scale and inclined plane test were used to evaluate the neurological function of the rats. Flow cytometric assay was performed to detect the apoptotic rates of cells in the spinal cord. ELISA and western blot analysis were performed to determine the levels of interleukin (IL)-10, tumor necrosis factor (TNF)-α, cyclophilin-D (Cyp-D) and apoptosis-inducing factor (AIF). The results demonstrated that CsA significantly improved the neurological function of the SCI rats with hyperglycemia. CsA markedly reduced the number of apoptotic cells exaggerated by hyperglycemia in the spinal cord of the SCI rats. CsA significantly decreased the expression levels of IL-10, TNF-α, Cyp-D and AIF in the spinal cord of the SCI rats. Overall, the present study revealed a significant role of CsA in the treatment of SCI in the presence of hyperglycemia by inhibiting the apoptosis of spinal cord cells.
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影响因子：
4.5
作者：
Alexanian, Arshak R.;Svendsen, Clive N.;Kurpad, Shekar N.
通讯作者：
Kurpad, Shekar N.
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影响因子：
4.2
作者：
Lau D;Harte SE;Morrow TJ;Wang S;Mata M;Fink DJ
通讯作者：
Fink DJ
影响因子：
4.2
作者：
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通讯作者：
Kim, Byung G.
影响因子：
2.9
作者：
Dai, Yuxiang;Sun, Qing;Shi, Jixin
通讯作者：
Shi, Jixin
影响因子：
1.2
作者：
Hashimoto, Akari;Kanisawa, Yuji;Kato, Junji
通讯作者：
Kato, Junji