Requirements of glycerol and fatty acid for triglyceride synthesis and ketogenesis by hepatocytes from normal and triiodothyronine-treated rats.
Requirements of glycerol and fatty acid for triglyceride synthesis and ketogenesis by hepatocytes from normal and triiodothyronine-treated rats.
复制标题
正常和三碘甲状腺原氨酸治疗大鼠的肝细胞合成甘油三酯和生酮所需的甘油和脂肪酸。
DOI：
10.1016/0006-291x(85)91906-0
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发表时间：
1985
影响因子：
3.1
通讯作者：
Heimberg,M
中科院分区：
文献类型：
--
作者：
Olubadewo,JO;Heimberg,M
与甲状腺功能正常动物的肝细胞相比，T3给药大鼠的肝细胞在0-2 mM的所有浓度下合成的甘油三酯较少，[1- 14 C]油酸酯的酮体较多;添加1.0 mM甘油可增加T3给药大鼠肝细胞的甘油三酯合成，并减少酮体生成，与未添加甘油时甲状腺功能正常肝细胞中观察到的速率相同。甘油没有改变甘油三酯的合成，但减少了甲状腺功能正常的肝细胞生酮。从这些和其它数据（J. Biol. Chem. 259，8857-8862（1985））中可以看出，在甲状腺机能亢进大鼠中，甘油-3-P而不是脂肪酸是甘油三酯合成的限速剂，其次是降低肝细胞中生酮速率的限速剂。
Hepatocytes from T3-treated rats synthesized less triglyceride and more ketone bodies from [1-14C]oleate at all concentrations from 0–2 mM, than did hepatocytes from euthyroid animals; addition of 1.0 mM glycerol increased triglyceride synthesis and reduced ketogenesis in hepatocytes from T3-treated rats to the rates observed in euthyroid hepatocytes in the absence of added glycerol. Glycerol did not alter triglyceride synthesis, but reduced ketogenesis by euthyroid hepatocytes. It is probable from these and other data (J. Biol. Chem. 259, 8857–8862 (1985)) that, in the hyperthyroid rat, glycero-3-P, and not fatty acid, is rate limiting for synthesis of triglyceride, and, secondarily for reducing rates of ketogenesis in the hepatocyte.