Pancreas development ex vivo: culturing embryonic pancreas explants on permeable culture inserts, with fibronectin-coated glass microwells, or embedded in three-dimensional Matrigel™.
Pancreas development ex vivo: culturing embryonic pancreas explants on permeable culture inserts, with fibronectin-coated glass microwells, or embedded in three-dimensional Matrigel™.
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DOI：
10.1007/978-1-4939-1435-7_17
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Sander, Maike
中科院分区：
文献类型：
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作者：
Shih, Hung Ping;Sander, Maike
胰腺发育是一个复杂而动态的过程，由细胞和分子事件（包括形态发生和细胞分化）精心策划。由于最近对可能的基于细胞疗法的糖尿病治疗的探索，研究人员在破译胰腺形成的发育程序方面取得了重大进展。体外胰腺器官培养系统为探索基因调控、细胞行为和细胞分化机制提供了有价值的工具。在本章中，我们回顾了培养胚胎胰腺外植体的三种常用技术。每种技术都适合不同的应用。具体来说，在培养插入物上培养胚胎胰腺提供了一个极好的平台来测试化合物的效果。相反，当胚胎胰腺在纤连蛋白包被的玻璃微孔中培养时，该系统提供独特的培养条件来监测器官生长和细胞动态事件。最后，当胚胎胰腺嵌入基质胶中时，可以在三维环境中研究器官发生，而不是将分析限制在一个平面上。
Pancreas development is a complex and dynamic process orchestrated by cellular and molecular events, including morphogenesis and cell differentiation. As a result of recent explorations into possible cell-therapy-based treatments for diabetes, researchers have made significant progress in deciphering the developmental program of pancreas formation. In vitro pancreas organ culture systems provide a valuable tool for exploring the mechanisms of gene regulation, cellular behaviors, and cell differentiation. In this chapter, we review three common techniques for culturing embryonic pancreas explants. Each technique is suitable for different applications. Specifically, culturing embryonic pancreas on culture inserts provides an excellent platform to test the effects of chemical compounds. Conversely, when the embryonic pancreas is cultured in fibronectin-coated glass microwells, the system provides unique culture conditions to monitor organ growth and cellular dynamic events. Lastly, when the embryonic pancreas is embedded in Matrigel, organogenesis can be studied in a three-dimensional environment instead of limiting the analysis to one plane.