Lipid composition in a strain of Bacillus subtilis, a producer of iturin A lipopeptides that are active against uropathogenic bacteria.
Lipid composition in a strain of Bacillus subtilis, a producer of iturin A lipopeptides that are active against uropathogenic bacteria.
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DOI：
10.1007/s11274-016-2126-0
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发表时间：
2016-10
影响因子：
4.1
通讯作者：
Chojniak J
中科院分区：
文献类型：
--
作者：
Bernat P;Paraszkiewicz K;Siewiera P;Moryl M;Płaza G;Chojniak J
尿路感染是人类常见的疾病。因此，需要新的方法来破坏由尿路病原体形成的生物膜。Iturin A脂肽（LPs）C14和C15是由枯草芽孢杆菌I′1a菌株合成的有效生物表面活性剂。通过检查来自I′1a培养物的提取物对从导尿管上的生物膜中分离的尿路致病菌的抑制作用，证实了提取的LP的生物活性。与DSM 3257相比，DSM 3257产生的表面活性素水平相对较低，从菌株I′1a获得的提取物对大肠杆菌、粘质沙雷氏菌、阴沟肠杆菌、奇异变形杆菌、弗氏柠檬酸杆菌和粪肠球菌的无菌和固着形式都表现出更大的抑制作用。此外，环状LP生物表面活性剂可能会干扰细胞质膜的完整性，因此，我们研究了合成的LP对B的脂肪酸和磷脂的影响。枯草杆菌。使用GC-MS、LC-MS/MS和MALDI-TOF/TOF技术分析LP和脂质。与B相比。枯草芽孢杆菌DSM 3257，I′1a菌株的膜的特征在于反异构脂肪酸的量增加，并且磷脂酰甘油（PG）与磷脂酰乙醇胺（PE）的比率高10倍。有趣的是，在B.补充有I′1a菌株的LP提取物的枯草芽孢杆菌DSM 3257，PG与PE的比率高4倍，并且反异构脂肪酸的量也增加。
Urinary tract infections are a common disease in humans. Therefore, new methods are needed to destroy biofilms that are formed by uropathogens. Iturin A lipopeptides (LPs) C14 and C15 are potent biosurfactants synthetized by the Bacillus subtilis I′1a strain. The biological activity of extracted LPs was confirmed by examining extracts from I′1a cultures against uropathogenic bacteria that had been isolated from biofilms on urinary catheters. Compared with cultures of DSM 3257, which produce surfactin at a relatively low level, the extract obtained from strain I′1a exhibited a greater inhibitory effect against both planktonic and sessile forms of Escherichia coli, Serratia marcescens, Enterobacter cloacae, Proteus mirabilis, Citrobacter freundii and Enterococcus faecalis. Moreover, cyclic LP biosurfactants may disturb the integrity of cytoplasmic membranes; therefore, we investigated the effects of synthetized LPs on fatty acids and phospholipids of B. subtilis. LPs and lipids were analyzed using GC–MS, LC–MS/MS and MALDI-TOF/TOF techniques. Compared with B. subtilis DSM 3257, membranes of the I′1a strain were characterized by an increased amount of anteiso fatty acids and a ten-fold higher ratio of phosphatidylglycerol (PG)-to-phosphatidylethanolamine (PE). Interestingly, in cultures of B. subtilis DSM 3257 supplemented with LP extracts of the I′1a strain, the PG-to-PE ratio was fourfold higher, and the amount of anteiso fatty acids was also increased.