Molecular analysis of microbial population transition associated with the start of denitrification in a wastewater treatment process
Molecular analysis of microbial population transition associated with the start of denitrification in a wastewater treatment process
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DOI：
10.1111/j.1365-2672.2005.02698.x
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发表时间：
2005-01-01
影响因子：
4
通讯作者：
Inamori, Y
中科院分区：
文献类型：
--
作者：
Hoshino, T;Terahara, T;Inamori, Y
目的：本研究的目的是通过监测反硝化过程中的分子动力学来确定在反硝化过程中起重要作用的细菌。方法和结果：用荧光标记的末端限制性片段长度多态性(T-RFLP)和克隆分析的非标记引物分别扩增16S rRNA反转录的cDNADNA。测定了随着反硝化开始而增加的末端限制性片段(T-RF)。通过对克隆获得的16S rRNA序列进行电子分析，鉴定了这些T-RFs。结论：针对16S rRNA的T-RFLP分析方法适用于污水处理过程中细菌群落的日常监测。结合T-RFLP分析结果和16S rRNA克隆文库，表明噬氢菌属和嗜酸杆菌属细菌在反硝化过程中起着重要的作用。研究的意义和影响：本研究结果为污水处理过程中活性反硝化细菌的16S rRNA水平提供了新的见解。
Aims; The objective of this study is to determine the bacteria playing an important role in denitrification by monitoring the molecular dynamics accompanying the start of denitrification.Methods and Results: cDNA reverse-transcribed from 16S rRNA was amplified with fluorescent labelled primer for terminal restriction fragment length polymorphism (T-RFLP) analysis and an unlabelled primer for cloning analysis. The terminal restriction fragments (T-RFs) that increased in association with the start of denitrification were determined. These T-RFs were identified by in silico analysis of 16S rRNA sequences obtained from cloning. As a result, it was clearly observed that the bacteria belonging to the genera Hydrogenophaga and Acidovorax increased in number after the start of denitrification.Conclusions: It was demonstrated that T-RFLP analysis targeting 16S rRNA is appropriate for the daily monitoring of a bacterial community to control wastewater treatment processes. Combination of the results of T-RFLP analysis and 16S rRNA clone library indicated that the bacteria belonging to the genera Hydrogenophaga and Acidovorax play an important role in denitrification.Significance and Impact of the Study: The results of this study provide new insight to the 16S rRNA level of active denitrifying bacteria in wastewater treatment processes.