Baicalein induces CD4(+)Foxp3(+) T cells and enhances intestinal barrier function in a mouse model of food allergy.
Baicalein induces CD4(+)Foxp3(+) T cells and enhances intestinal barrier function in a mouse model of food allergy.
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DOI：
10.1038/srep32225
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发表时间：
2016-08-26
影响因子：
4.6
通讯作者：
Shon DH
中科院分区：
文献类型：
--
作者：
Bae MJ;Shin HS;See HJ;Jung SY;Kwon DA;Shon DH
食物过敏的发病率在全球范围内呈逐年上升趋势。食物过敏是由过敏原渗入胃肠道，随后由T辅助细胞(Th)2介导的免疫反应引起的。通过对食物过敏模型小鼠肠道紧密连接的调节，研究了黄芩素(5，6，7-三羟基黄酮)对食物过敏小鼠调节性T(Treg)细胞诱导和肠道屏障功能的影响。用卵清蛋白口服激发小鼠的过敏反应，分析有无黄芩素对小鼠肠系膜淋巴结的过敏症状发生率和T细胞相关活性的影响。结果表明，黄芩素可明显改善小鼠食物过敏症状，降低血清IgE和效应性T细胞。然而，黄芩素上调Treg相关因子的表达。此外，黄芩素可通过调节紧密连接来增强肠道屏障功能。我们还发现，黄芩素通过芳香烃受体(AHRs)诱导Treg细胞分化。因此，黄芩素作为AhR的激动剂可以诱导Treg分化，增强屏障功能，提示黄芩素可能是一种有效的食物来源的免疫调节剂，用于治疗食物过敏。
The incidence of food allergy, which is triggered by allergen permeation of the gastrointestinal tract followed by a T-helper (Th) 2-mediated immune response, has been increasing annually worldwide. We examined the effects of baicalein (5,6,7-trihydroxyflavone), a flavonoid from Scutellaria baicalensis used in oriental herbal medicine, on regulatory T (Treg) cell induction and intestinal barrier function through the regulation of tight junctions in a mouse model of food allergy. An allergic response was induced by oral challenge with ovalbumin, and the incidence of allergic symptoms and T cell-related activity in the mesenteric lymph nodes were analyzed with and without the presence of baicalein. Our results demonstrated that the administration of baicalein ameliorated the symptoms of food allergy and attenuated serum IgE and effector T cells. However, Treg-related factors were up-regulated by baicalein. Furthermore, baicalein was shown to enhance intestinal barrier function through the regulation of tight junctions. We also found that baicalein treatment induced the differentiation of Treg cells via aryl hydrocarbon receptors (AhRs). Thus, the action of baicalein as an agonist of AhR can induce Treg differentiation and enhance barrier function, suggesting that baicalein might serve as an effective immune regulator derived from foods for the treatment of food allergy.