Dentin demineralization: Effects of dentin depth, pH and different acids
Dentin demineralization: Effects of dentin depth, pH and different acids
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DOI：
10.1016/s0109-5641(97)80104-2
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发表时间：
1997-09-01
期刊：
影响因子：
5
通讯作者：
Marshall, SJ
中科院分区：
文献类型：
--
作者：
Marshall, GW;Inai, N;Marshall, SJ
目标.本研究试图确定：1）牙本质脱矿率是否与磷酸中脱矿的酸浓度成正比（10%或1.76M，0.025M，0.0001M，pH=0.95，2.0，4.0）：2）酸蚀特性是否与牙本质深度无关;以及3）在相似的pH范围内，磷酸的蚀刻特性是否与柠檬酸的蚀刻特性相当。高度抛光的牙本质盘从新鲜提取，非龋，第三磨牙准备与参考层。从浅或深冠状牙本质制备样品。在原子力显微镜（AFM）的湿池中使用磷酸溶液（pH = 0.95、2.0、4.0）蚀刻样品长达30分钟的时间。确定管间和管周牙本质相对于参考层的深度变化，以量化结构变化。将结果与使用柠檬酸（pH = 1.0、2.15或3.4）的类似研究进行比较。蚀刻特征用双向重复测量方差分析（p < 0.05）和Tukey多重比较检验进行统计学比较。管周牙本质的退缩与时间呈线性关系。在pH = 2.0和4.0的蚀刻溶液中，管间牙本质衰退迅速开始，但随后在非常短的时间内达到平台。在最高浓度下，衰退随着时间的推移而减少，但没有建立明确的平台。在任何浓度的磷酸溶液中，牙本质表面浅部和深部的小管周围酸蚀速率之间均无统计学差异。在牙本质深度不同的平台位置，牙本质管间退缩也没有差异。磷酸和柠檬酸的蚀刻速率随pH值的降低而显著增加，但柠檬酸的蚀刻速率高于磷酸。BFM允许量化在牙本质蚀刻过程中的变化。正如预期的那样，小管周围牙本质蚀刻率随着pH值的降低而增加，但变化不是线性的，并且在相似的pH值范围内研究的两种酸是不同的。管间牙本质表面凹陷较小，且在低浓度下趋于稳定，管周酸蚀速率和管间牙本质凹陷与牙本质深度无关。
Objectives. This investigation sought to determine: 1) if dentin demineralization rates are proportional to acid concentration for demineralization in phosphoric acid (10% or 1.76M, 0.025M, 0.0001M, with pH=0.95, 2.0, 4.0 respectively); 2) if the etching characteristics are independent of dentin depth; and 3) if the etching characteristics for phosphoric acid were comparable to those for citric acid over a similar pH range.Methods. Highly polished dentin disks from freshly extracted, non-carious, third molars were prepared with a reference layer. Samples were prepared from either superficial or deep coronal dentin. The samples were etched for periods of up to 30 min using phosphoric acid solutions (pH = 0.95, 2.0, 4.0) in a wet cell of an atomic force microscope (AFM). Depth changes with respect to the reference layer were determined for the intertubular and peritubular dentin to quantify structural changes. The results were compared with similar studies using citric acid (pH = 1.0, 2.15 or 3.4). Etching characteristics were statistically compared using 2-way repeated measures ANOVA at p < 0.05 and the Tukey's multiple comparison test.Results. The relation between time and recession for peritubular dentin was initially linear. The intertubular dentin recession started rapidly but then reached a plateau within a very short interval for etching solutions at pH = 2.0 and 4.0. At the highest concentration, the recession decreased with time, but a clear plateau was not established. There was no statistical difference between peritubular etching rates of superficial and deep dentin surfaces with phosphoric acid at any concentration. There was also no difference in the intertubular dentin recession at the location of the plateau that depended on dentin depth. Etching rates increased dramatically with decreased pH for both phosphoric and citric acids, but were higher for citric acid than for phosphoric acid.Significance. The BFM allowed quantification of changes during etching of we dentin. Peritubular dentin etching rates increased with decreasing pH, as expected, but changes were not linear and were different for the two acids studied over a similar pH range. Intertubular dentin surface recession was small and plateaued for low concentrations, The peritubular etching rate and intertubular dentin recession did not depend on dentin depth.