ENDOTHELIUM-DEPENDENT CONTRACTIONS TO CALCIUM IONOPHORE-A23187, ARACHIDONIC-ACID, AND ACETYLCHOLINE IN CANINE BASILAR ARTERIES
ENDOTHELIUM-DEPENDENT CONTRACTIONS TO CALCIUM IONOPHORE-A23187, ARACHIDONIC-ACID, AND ACETYLCHOLINE IN CANINE BASILAR ARTERIES
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DOI：
10.1161/01.str.19.4.476
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发表时间：
1988-04-01
期刊：
影响因子：
8.3
通讯作者：
VANHOUTTE, PM
中科院分区：
文献类型：
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作者：
KATUSIC, ZS;SHEPHERD, JT;VANHOUTTE, PM
本文研究了钙离子载体A23187、花生四烯酸和乙酰胆碱对犬离体基动脉的作用。有内皮和无内皮的环悬吊在生理盐水中进行等长张力记录。在非刺激环中，A23187、花生四烯酸和乙酰胆碱引起内皮依赖性收缩。去内皮对5‘’-三磷酸尿苷引起的血管环收缩无影响。环氧合酶抑制剂吲哚美辛(10-5M)可阻止对A23187、花生四烯酸和乙酰胆碱的兴奋反应，但不能改变KCl引起的收缩。血栓素合成酶抑制剂达唑西本(10-4M)显著减少A23187和花生四烯酸的内皮依赖性收缩，但对乙酰胆碱引起的收缩无明显影响。这些结果表明，A23187、花生四烯酸和乙酰胆碱通过促进内皮细胞释放环氧合酶产物(S)而引起犬脑血管兴奋性内皮依赖性反应，而A23187和花生四烯酸则通过血栓烷A2参与内皮依赖性收缩。
The effects of the calcium ionophore A23187, arachidonic acid, and acetylcholine were studied in isolated canine basilar arteries. Rings with and without endothelium were suspended for isometric tension recording in physiological saline. In unstimulated rings, A23187, arachidonic acid, and acetylcholine caused endothelium-dependent contractions. The contractions of rings caused by uridine 5''-triphosphate were not affected by removal of the endothelium. An inhibitor of cyclooxygenase, indomethacin (10-5 M), prevented excitatory responses to A23187, arachidonic acid, and acetylcholine but did not alter contractions caused by KCl. An inhibitor of thromboxane synthetase, dazoxiben (10-4 M), significantly reduced endothelium-dependent contractions to A23187 and arachidonic acid but did not significantly affect contractions caused by acetylcholine. These results demonstrate that A23187, arachidonic acid, and acetylcholine cause excitatory endothelium-dependent responses in canine cerebral blood vessels by increasing the release of product(s) of cyclooxygenase from endothelial cells; in the case of A23187 and arachidonic acid, thromboxane A2 contributes to the endothelium-dependent contractions.