The posterior determinant gene nanos is required for the maintenance of the adult germline stem cells during Drosophila oogenesis.
The posterior determinant gene nanos is required for the maintenance of the adult germline stem cells during Drosophila oogenesis.
复制标题
后决定基因 nanos 是果蝇卵子发生过程中成体生殖干细胞维持所必需的。
DOI：
10.1093/genetics/151.4.1479
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发表时间：
1999
期刊：
影响因子：
3.3
通讯作者：
Bhat,KM
中科院分区：
文献类型：
--
作者：
Bhat,KM
In a variety of tissues in eukaryotes, multipotential stem cells are responsible for maintaining a germinal population and generating a differentiated progeny. The Drosophila germline is one such tissue where a continuous supply of eggs or sperm relies on the normal functioning of stem cells. Recent studies have implicated a possible role for the posterior determinant gene nanos (nos) in stem cells. Here, I report that nanos is required in the Drosophila female germline as well as in the male germline. In the female, nos is required for the functioning of stem cells. In nos mutants, while the stem cells are specified, these cells divide only a few times at the most and then degenerate. The loss of germline stem cells in nos mutant mothers appears to be due to a progressive degeneration of the plasma membrane. Furthermore, following germ cell loss, the germaria in the nos mutant mothers appear to carry on massive mitochondrial biogenesis activity. Thus, the syncytia of such germaria are filled with mitochondria. In the male germline, the male fertility assay indicates that nos appears to be also required for the maintenance of stem cells. In these mutant males, spermatogenesis is progressively affected and these males eventually become sterile. These results indicate novel requirements for nos in the Drosophila germline.
