In vitro and in vivo effects of a novel bioactive glass-based cement used as a direct pulp capping agent
In vitro and in vivo effects of a novel bioactive glass-based cement used as a direct pulp capping agent
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DOI：
10.1002/jbm.b.34107
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发表时间：
2019-01-01
影响因子：
3.4
通讯作者：
Kitamura, Chiaki
中科院分区：
文献类型：
--
作者：
Hanada, Kaori;Morotomi, Takahiko;Kitamura, Chiaki
直接盖髓是保存牙髓活力的重要方法。盖髓剂必须具有若干性质，包括可用性、生物相容性和诱导修复性牙本质形成的能力。在这项研究中，一种新的生物活性玻璃基水泥进行了检查，以确定是否有必要的性能，作为一个直接盖髓剂的水泥。分析了生物活性玻璃基骨水泥的物理化学性质和骨水泥对成牙本质细胞样细胞的体外作用，以及对暴露牙髓的体内作用。将骨水泥浸泡在pH 10的水中，在模拟体液中，在骨水泥表面诱导羟基磷灰石样沉淀。对成牙本质细胞样细胞的活力、碱性磷酸酶活性或钙沉积能力没有细胞毒性作用。在暴露牙髓模型的体内大鼠研究中，骨水泥通过在牙髓暴露区域表达成牙本质细胞标记的成牙本质细胞样细胞诱导足够水平的修复性牙本质形成。这些结果表明，新开发的生物活性玻璃基水泥提供了良好的生物相容性与牙髓，并可能是有用的直接盖髓剂。(c)2018 Wiley Periodicals，Inc. J Biomed Mater Res Part B：Appl Biomater，107 B：161-168，2019。
Direct pulp capping is an important procedure for preserving pulp viability. The pulp capping agent must possess several properties, including usability, biocompatibility, and the ability to induce reparative dentin formation. In this study, a novel bioactive glass-based cement was examined to determine whether the cement has the necessary properties to act as a direct pulp capping agent. Physicochemical properties of the bioactive glass-based cement and in vitro effects of the cement on odontoblast-like cells, as well as in vivo effects on the exposed dental pulp, were analyzed. The cement immersed in water stabilized at pH10, and hydroxyapatite-like precipitation was induced on the surface of the cement in simulate body fluid. There were no cytotoxic effects on the viability, alkaline phosphatase activity, or calcium deposition ability of odontoblast-like cells. In the in vivo rat study of an exposed dental pulp model, the cement induced a sufficient level of reparative dentin formation by odontoblast-like cells expressing odontoblastic markers at the exposed area of the dental pulp. These results suggest that the newly developed bioactive glass-based cement provides favorable biocompatibility with the dental pulp and may be useful as a direct pulp capping agent. (c) 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 107B: 161-168, 2019.