Lucigenin (Bis-N-methylacridinium) as a mediator of superoxide anion production
Lucigenin (Bis-N-methylacridinium) as a mediator of superoxide anion production
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DOI：
10.1006/abbi.1997.9766
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发表时间：
1997-01-01
影响因子：
3.9
通讯作者：
Fridovich, I
中科院分区：
文献类型：
--
作者：
Liochev, SI;Fridovich, I
光亮素(双-N-甲基吖啶)(Luc(2+))常用于O-2(-)的发光检测。事实上，导致这种发光的途径需要LUC(2+)单价还原为LUCH(.+)，然后LUCH(.+)与O-2(-)反应生成不稳定的二氧杂环己烷。现在表明，LUCH(.+)迅速自氧化，从而生成(-)(2)。因此，Luc(2+)可以在葡萄糖和葡萄糖氧化酶等体系中介导O-2(-)的产生，在该体系中通常不产生O-2(-)。因此，Luc(2+)发光可以作为测定超氧化物歧化酶活性的基础，但不能用于测量甚至检测O-2(-)。(C)1997年学术出版社，Inc.
Lucigenin (bis-N-methylacridinium) (LUC(2+)) has frequently been used for the luminescent detection of O-2(-). In fact, the pathway leading to this luminescence requires univalent reduction of Luc(2+) to LucH(.+) followed by formation of an unstable dioxetane by reaction of LucH(.+) with O-2(-). It is now shown that LucH(.+) rapidly autooxidizes and so produces (-)(2). Luc(2+) can thus mediate O-2(-) production in systems, such as glucose plus glucose oxidase, in which there is ordinarily no O-2(-) production. Luc(2+) luminescence can thus be used as the basis for assaying superoxide dismutase activity but should not be used for measuring, or even detecting, O-2(-). (C) 1997 Academic Press, Inc.