Genome-wide miRNA profiling of villus and decidua of recurrent spontaneous abortion patients
Genome-wide miRNA profiling of villus and decidua of recurrent spontaneous abortion patients
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复发性流产患者绒毛和蜕膜的全基因组 miRNA 分析
DOI：
10.1530/rep-14-0095
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发表时间：
2014-07-01
期刊：
影响因子：
3.8
通讯作者：
Zhang, Jinping
中科院分区：
文献类型：
--
作者：
Dong, Fulu;Zhang, Yuan;Zhang, Jinping
MicroRNAs(MiRNAs)是一类约22个核苷酸的非编码RNA分子，参与转录后基因调控。有证据表明，miRNAs在子宫内膜异位症、子痫前期、不孕症等生殖系统疾病中起重要作用。然而，miRNAs是否与复发性自然流产(RSA)有关尚不清楚。在这项工作中，我们使用人miRNA芯片分析了RSA患者和正常妊娠(NP)妇女六对绒毛或蜕膜中miRNA的表达谱。部分芯片检测结果经RT-qPCR证实。RSA患者绒毛组织中hsa-miR-184、hsa-miR-187和hsa-miR-125b-2的表达明显高于正常对照组，而hsa-miR-520F、hsa-miR-3175和hsa-miR-4672的表达明显低于正常对照组。RSA患者蜕膜中共有5个miRNAs(hsa-miR-517c、hsa-miR-519a-1、hsa-miR-522、hsa-miR-520h和hsa-miR-184)表达上调。通过miRWalk对这些差异表达的miRNAs的靶基因进行预测，我们推测存在一个通过靶基因调控RSA的miRNA网络，这些靶基因在黏附、凋亡和血管生成方面发挥作用。我们的研究有助于阐明RSA发生发展的分子机制，并为今后的研究提供参考。
MicroRNAs (miRNAs) are non-coding RNA molecules of about 22 nucleotides that involved in post-transcriptional gene regulation. Evidence indicates that miRNAs play essential roles in endometriosis, pre-eclampsia, infertility and other reproductive system diseases. However, whether miRNAs are involved in recurrent spontaneous abortion (RSA) is unclear. In this work, we analysed the miRNA expression profiles in six pairs of villus or decidua from RSA patients and normal pregnancy (NP) women using a human miRNA microarray. Some of the chip results were confirmed by RT-qPCR. In the villi of RSA patients, expression of hsa-miR-184, hsa-miR-187 and hsa-miR-125b-2 was significantly higher, while expression of hsa-miR-520f, hsa-miR-3175 and hsa-miR-4672 was significantly lower, comparing with those of NP control. As well, a total of five miRNAs (hsa-miR-517c, hsa-miR-519a-1, hsa-miR-522, hsa-miR-520h and hsa-miR-184) were upregulated in the decidua of RSA patients. The target genes of these differentially expressed miRNAs were predicted by miRWalk, and we speculate a network of miRNA regulating RSA by target genes function on adhesion, apoptosis and angiogenesis. Our study may help clarify the molecular mechanisms which are involved in the progression of RSA, and provide a reference for future research.