Molecular, Topographic, and Functional Organization of the Cerebellar Nuclei: Analysis by Three-Dimensional Mapping of the Olivonuclear Projection and Aldolase C Labeling
Molecular, Topographic, and Functional Organization of the Cerebellar Nuclei: Analysis by Three-Dimensional Mapping of the Olivonuclear Projection and Aldolase C Labeling
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DOI：
10.1523/jneurosci.1579-07.2007
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发表时间：
2007-09
期刊：
影响因子：
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通讯作者：
I. Sugihara;Y. Shinoda
中科院分区：
文献类型：
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作者：
I. Sugihara;Y. Shinoda
橄榄小脑攀爬纤维投射模式与小脑浦肯野细胞醛缩酶C表达模式密切相关。根据这种表达模式，橄榄小脑投射可以分为五个功能区。每组起源于下橄榄内的一个分区，该分区投射到浦肯野细胞的多个皮质条纹，所有这些条纹都是醛缩酶C阳性或醛缩酶C阴性。然而，在小脑核（CN）中没有类似的房室组织。因此，在CN的大鼠，我们系统地映射属于五个组的橄榄球预测的位置，并确定它们的关系，在浦肯野细胞轴突终末的醛缩酶C的表达。CN分为尾腹侧醛缩酶C阳性和吻背侧醛缩酶C阴性两部分。橄榄球终端从五个组投影地形五个独立的车厢内CN部分跨越传统的边界，定义的顶，插入，和齿状核。每个隔室有大部分均匀的细胞结构和相同的醛缩酶C表达（阳性或阴性），发现在相应的橄榄皮质投影。这些结果提出了一个新的观点的组织CN，橄榄球终端的模式链接部分不同CN在一起。我们建议CN中的每个隔间，沿着与其相应的橄榄亚区和皮质条纹，可能与运动控制的不同方面。
The olivocerebellar climbing fiber projection pattern is closely correlated with the pattern of aldolase C expression in cerebellar Purkinje cells. Based on this expression pattern, the olivocerebellar projection can be classified into five “groups” of functional compartments. Each group originates from a subarea within the inferior olive that projects to multiple cortical stripes of Purkinje cells, all of which are either aldolase C positive or aldolase C negative. However, no equivalent compartmental organization has been demonstrated in the cerebellar nuclei (CN). Thus, in the CN of the rat, we systematically mapped the location of olivonuclear projections belonging to the five groups and determined their relationship to the expression of aldolase C in Purkinje cell axonal terminals. The CN were divided into caudoventral aldolase C-positive and rostrodorsal aldolase C-negative parts. The olivonuclear terminations from the five groups projected topographically to five separate compartments within the CN that partly crossed the traditional boundaries that define the fastigial, interposed, and dentate nuclei. Each compartment had mostly uniform cytoarchitecture and the same aldolase C expression (either positive or negative) that was found in the corresponding olivocortical projection. These results suggest a new view of the organization of the CN whereby the pattern of olivonuclear terminations links portions of different CN together. We propose that each compartment in the CN, along with its corresponding olivary subarea and cortical stripes, may be related to a different aspect of motor control.