Pressure acceleration of proteolysis: A general mechanism
Pressure acceleration of proteolysis: A general mechanism
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蛋白水解的压力加速：一般机制
DOI：
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发表时间：
2008
期刊：
影响因子：
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通讯作者：
Ken Sasaki
中科院分区：
文献类型：
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作者：
K. Akasaka;H. Nagahata;A. Maeno;Ken Sasaki
以泛素为底物，α-糜蛋白酶为酶，在kbar范围内，压力对酶促蛋白水解有显著的促进作用。这种加速是由于压力使泛素的构象平衡从不可降解的折叠构象转变为酶可降解的未折叠构象，而α-糜蛋白酶的酶活性在很大程度上得以保留。这种机制被认为是普遍适用于大多数球状蛋白质和压力加速的蛋白质水解的方法将有一个巨大的潜在效用的系统中，需要有效地去除蛋白质。
Remarkable acceleration of enzymatic proteolysis by pressure at kbar range is reported with ubiquitin as substrate and α-chymotrypsin as enzyme. The acceleration is interpreted in terms of the shift of conformational equilibrium in ubiquitin from the non-degradable folded conformer to the enzyme-degradable unfolded conformer by pressure because of the lower volume of the latter, while the enzymatic activity of α-chymotrypsin is still largely retained. This mechanism is considered generally applicable to most globular proteins and the method of pressure-accelerated proteolysis will have an enormous potential utility in systems wherever efficient removal of proteins is needed.
影响因子：
5.6
作者：
VIJAYKUMAR, S;BUGG, CE;COOK, WJ
通讯作者：
COOK, WJ