Mosaic-like structure of penicillin-binding protein 2 gene (PenA) in clinical isolates of Neissetia gonorrhoeae with reduced susceptibility to cefixime
Mosaic-like structure of penicillin-binding protein 2 gene (PenA) in clinical isolates of Neissetia gonorrhoeae with reduced susceptibility to cefixime
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DOI：
10.1128/aac.46.12.3744-3749.2002
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发表时间：
2002-12-01
影响因子：
4.9
通讯作者：
Oishi, Y
中科院分区：
文献类型：
--
作者：
Ameyama, S;Onodera, S;Oishi, Y
2000年和2001年，从日本东京的男性尿道炎患者中分离出对头孢克肟敏感性降低的淋球菌菌株（MIC，0.25 - 0.5 μ g/ml）。对头孢克肟、青霉素等头孢烯类抗生素的耐药性转移到敏感受体N.通过转化青霉素结合蛋白2基因（佩纳A）转化淋球菌ATCC 19424，该基因已通过PCR从对头孢克肟敏感性降低的菌株中扩增（MIC，0.5 μ g/ml）。对头孢克肟敏感性降低的菌株的佩纳序列与其他敏感菌株的序列不同，与已报道的N.淋病佩纳基因序列。该佩纳基因的转肽酶编码区中的某些区域与全黄奈瑟氏菌（N. sicca）、灰奈瑟氏菌、黄奈瑟氏菌和脑膜炎奈瑟氏菌。这些结果表明，佩纳基因中的镶嵌样结构降低了对N.淋病奈瑟菌对头孢霉素和青霉素的敏感性与淋病奈瑟菌相似。脑膜炎和肺炎链球菌。
Neisseria gonorrhoeae strains with reduced susceptibility to cefixime (MICs, 0.25 to 0.5 mug/ml) were isolated from male urethritis patients in Tokyo, Japan, in 2000 and 2001. The resistance to cephems including cefixime and penicillin was transferred to a susceptible recipient, N. gonorrhoeae ATCC 19424, by transformation of the penicillin-binding protein 2 gene (penA) that had been amplified by PCR from a strain with reduced susceptibility to cefixime (MIC, 0.5 mug/ml). The sequences of penA in the strains with reduced susceptibilities to cefixime were different from those of other susceptible isolates and did not correspond to the reported N. gonorrhoeae penA gene sequences. Some regions in the transpeptidase-encoding domain in this penA gene were similar to those in the penA genes of Neisseria perflava (N. sicca), Neisseria cinerea, Neisseria flavescens, and Neisseria meningitidis. These results showed that a mosaic-like structure in the penA gene conferred reductions in the levels of susceptibility of N. gonorrhoeae to cephems and penicillin in a manner similar to that found for N. meningitidis and Streptococcus pneumoniae.