Hemoglobin A1 in hemolysates from healthy and insulin-dependent diabetic children, as determined with a temperature-controlled minicolumn assay.
Hemoglobin A1 in hemolysates from healthy and insulin-dependent diabetic children, as determined with a temperature-controlled minicolumn assay.
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通过温控微柱测定测定健康儿童和胰岛素依赖型糖尿病儿童的溶血产物中的血红蛋白 A1。
DOI：
10.1093/clinchem/28.1.13
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发表时间：
1982
影响因子：
9.3
通讯作者：
E. Stein
中科院分区：
文献类型：
--
作者：
L. Kaplan;D. Cline;P. Gartside;S. Burstein;M. Sperling;E. Stein
我们报告了非糖尿病婴儿、儿童和年轻人的年龄特异性糖化血红蛋白值。这些值用离子交换“微型柱”在特别设计和构造的水浴中测定，该水浴将柱温保持在23 +/- 0.1 ℃。使用两种内部制备的质控品（糖化血红蛋白含量分别为总血红蛋白的6.36%和11.87%，储存于-20 ℃）评估长期分析精密度。日间精密度（CV）分别为1.4%和1.65%。我们发现129名3-23岁胰岛素依赖型糖尿病患者的糖化血红蛋白值与医生对临床控制的独立评估之间存在显著相关性（r = 0.981，p <0.01）。糖尿病控制良好或控制不佳的患者与控制中等的患者之间的糖化血红蛋白值存在显著差异（p <0.05）。对20名患者进行的3 - 12周的研究报告显示，临床控制的变化被糖化血红蛋白值的变化所掩盖。
We report age-specific glycohemoglobin values for nondiabetic infants, children, and young adults. These values were determined with an ion-exchange "mini-column" in a specially designed and constructed water bath that maintains column temperature at 23 +/- 0.1 degrees C. Two in-house-prepared controls with glycohemoglobin content amounting to 6.36% and 11.87% of total hemoglobin, stored at -20 degrees C, were used to assess long-term analytical precision. Between-day precision (CV) was 1.4% and 1.65%, respectively. We found a significant correlation (r = 0.981, p less than 0.01) between the glycohemoglobin value and the physicians' independent assessment of clinical control in 129 insulin-dependent diabetics, ages 3-23 years. There were significant differences (p less than 0.05) between glycohemoglobin values between patients with well-controlled or poorly controlled diabetes and those with intermediate control. Reported studies of 20 patients over three to 12 weeks showed that changes in clinical control were paralleled by changes in glycohemoglobin values.