Maternal sodium butyrate supplement elevates the lipolysis in adipose tissue and leads to lipid accumulation in offspring liver of weaning-age rats.
Maternal sodium butyrate supplement elevates the lipolysis in adipose tissue and leads to lipid accumulation in offspring liver of weaning-age rats.
复制标题
母体丁酸钠补充会提高脂肪组织中的脂解作用并导致断奶大鼠子代肝脏中的脂质积累
DOI：
10.1186/s12944-016-0289-1
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发表时间：
2016-07-22
影响因子：
4.5
通讯作者：
Yang X
中科院分区：
文献类型：
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作者：
Zhou J;Gao S;Chen J;Zhao R;Yang X
研究背景丁酸钠（sodium butyrate，SB）对哺乳动物脂代谢的调节作用，研究其与子代生长发育的关系，为探讨SB对断奶大鼠肝脏脂代谢的影响，我们对16只SD雌性大鼠在妊娠期和哺乳期分别饲喂普通饲料和1%丁酸钠饲料。在断奶年龄，母亲的皮下脂肪组织和后代的肝脏样品。结果母鼠补充SB后，母鼠和仔鼠血清中非酯化脂肪酸（NEFA）浓度均显著升高（P <0. 05），而对照组血清中NEFA浓度显著升高（P<0. 05）。断乳大鼠血清总胆固醇（Tch）明显升高（P< 0.05）。补充SB的大鼠母体脂肪组织中蛋白G偶联蛋白（GPR43）和蛋白激酶A（PKA）的含量显著升高（P< 0.05）。与对照组相比，母体脂肪组织中蛋白质脂肪甘油三酯脂肪酶（ATGL）、总脂肪酶和磷酸化激素敏感脂肪酶（HSL）的表达显著增加（P< 0.05）。而与脂肪生成相关的蛋白质则无明显变化。仔鼠肝脏中甘油三酯浓度显著升高，这可能与脂肪酸结合蛋白（FABP）和脂肪酸转位酶（CD36）蛋白水平升高有关（P< 0.05）。妊娠期和哺乳期SB暴露增加肝脏总胆固醇（Tch）含量（P< 0.01），这与子代肝脏低密度脂蛋白受体（LDLR）蛋白表达显著上调有关结论妊娠期和哺乳期补充SB可促进母体脂肪动员，这可能导致后代肝脏中的脂肪酸摄取和脂质积累。
BackgroundSodium butyrate (SB) is reported to regulate lipid metabolism in mammals, and the relationship between maternal nutrition and offspring growth has drawn much attention in the last several years.MethodsTo elucidate the effects of maternal dietary SB supplementation on hepatic lipid metabolism in weaning rats, we fed 16 primiparous purebred female SD rats either a chow-diet or a 1 % sodium butyrate diet throughout pregnancy and lactation. At weaning age, samples of the maternal subcutaneous adipose tissue and offspring liver were taken. The serum indexes and expressions of proteins related to lipid metabolism were detected in the mother and offspring, respectively.ResultsThe results showed that the maternal SB supplement increased the concentration of non-esterified fatty acid (NEFA) in the maternal and offspring serum (P< 0.05). Total cholesterol (Tch) increased significantly in the weaning-rat serum (P< 0.05). Maternal adipose tissue from the SB-supplemented rats showed higher content of protein G-coupled protein (GPR43) and protein kinase A (PKA) (P< 0.05). The expression of protein adipose triglyceride lipase (ATGL), and of total and phosphorylated hormone sensitive lipase (HSL), in the maternal adipose tissue increased significantly (P< 0.05) compared to the control group. However the proteins related to lipogenesis showed no significant changes. Moreover, the concentration of triglyceride in the offspring liver increased significantly, and this likely resulted from an increase in the levels of fatty acids binding protein (FABP) and fatty acid translocase (CD36) protein (P< 0.05). SB exposure during pregnancy and lactation increased the hepatic total cholesterol (Tch) content (P< 0.01), which was related to a significantly up-regulated offspring hepatic expression of low density lipoprotein receptor (LDLR) protein (P< 0.05).ConclusionThese results indicate that a maternal SB supplement during pregnancy and the lactation period promotes maternal fat mobilization, which may result in fatty acid uptake and lipid accumulation in the liver of the offspring.