Expression of cartilage-related genes in bovine synovial tissue.
Expression of cartilage-related genes in bovine synovial tissue.
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牛滑膜组织中软骨相关基因的表达。
DOI：
10.1002/jor.20345
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发表时间：
2007
期刊：
影响因子：
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通讯作者：
Hunziker,ErnstB
中科院分区：
文献类型：
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作者：
Shintani,Nahoko;Kurth,Tobias;Hunziker,ErnstB
滑膜含有具有软骨形成潜能的间充质干细胞。虽然滑膜和关节软骨组织是从一个共同的间充质细胞库发展而来的，但对它们的遗传共性知之甚少。在本研究中，使用真实的时间聚合酶链反应测量了几种软骨相关蛋白的mRNA水平，即软骨寡聚基质蛋白（COMP）、Sox 9、聚集蛋白聚糖和I型、II型、IX型、X型和XI型胶原。我们的数据显示，小腿掌骨关节滑膜生理表达不仅是I型胶原，但也COMP，Sox 9，聚集蛋白聚糖，和胶原类型X和XI。后五种蛋白的mRNA水平在关节软骨中的2%至15%之间。我们推测这些基因是由软骨祖细胞表达的，其在滑膜中的存在反映了该组织和关节软骨在胎儿发育中的共同个体发育阶段。© 2007骨科研究学会。出版社：Wiley Periodicals，Inc. J Orthop Res 25：813-819，2007
The synovium contains mesenchymal stem cells with chondrogenic potential. Although synovial and articular cartilage tissue develop from a common pool of mesenchymal cells, little is known about their genetic commonalities. In the present study, the mRNA levels for several cartilage‐related proteins, namely, cartilage oligomeric matrix protein (COMP), Sox9, aggrecan, and collagen types I, II, IX, X, and XI, were measured using the real‐time polymerase chain reaction. Our data reveal the synovium of calf metacarpal joints to physiologically express not only type I collagen but also COMP, Sox9, aggrecan, and collagen types X and XI. The mRNA levels for the latter five proteins lie between 2% and 15% of those in articular cartilage. We speculate that these genes are being expressed by chondroprogenitor cells, whose presence in the synovium reflects a common ontogenetic phase in the fetal development of this tissue and of articular cartilage. © 2007 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 25: 813–819, 2007