G protein gamma subunit modulates expression of plant-biomass-degrading enzyme genes and mycelial-development-related genes in Penicillium oxalicum
G protein gamma subunit modulates expression of plant-biomass-degrading enzyme genes and mycelial-development-related genes in Penicillium oxalicum
复制标题
G蛋白γ亚基调节草酸青霉植物生物质降解酶基因和菌丝体发育相关基因的表达
DOI：
10.1007/s00253-021-11370-3
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发表时间：
2021
影响因子：
5
通讯作者：
Shuai Zhao
中科院分区：
文献类型：
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作者：
Xiao-Ming Pao;Di Tian;Ting Zhang;Lu-Sheng Liao;Cheng-Xi Li;Xue-Mei Luo;Jia-Xun Feng;Shuai Zhao
异三聚体G蛋白介导的信号通路在转录水平上调节真菌许多基本细胞过程中必需基因的表达。然而，这些过程仍然不清楚在青霉。在这项研究中，我们产生了gng-1（POX 07071）编码Gγ蛋白的敲除和敲除互补菌株，并发现GNG-1调节编码植物生物量降解酶（PBDEs）和孢子形成相关激活剂的基因的表达。有趣的是，GNG-1影响了thecxrB的表达，该基因编码一种已知的转录因子，该因子是主要纤维素酶和木聚糖酶基因表达所必需的。组成性过表达cxrBin GNG-1规避了PBDE生产对GNG-1的依赖性。进一步的证据表明，CxrB通过控制调控基因amyR的表达，间接调控关键淀粉酶基因的转录水平。这些数据扩展了Gγ蛋白功能的多样性，为丝状真菌中PBDE基因表达的信号转导和调控提供了新的见解。关键点·GNG-1调控PBDE基因和孢子形成相关基因的表达。·GNG-1控制关键调节基因cxrB的表达。·cxrB的过度表达避免了多溴二苯醚生产对GNG-1的依赖。
Heterotrimeric-G-protein-mediated signaling pathways modulate the expression of the essential genes in many fundamental cellular processes in fungi at the transcription level. However, these processes remain unclear inPenicillium oxalicum. In this study, we generated knockout and knockout-complemented strains ofgng-1(POX07071) encoding the Gγ protein and found that GNG-1 modulated the expression of genes encoding plant-biomass-degrading enzymes (PBDEs) and sporulation-related activators. Interestingly, GNG-1 affected expression of thecxrBthat encodes a known transcription factor required for the expression of major cellulase and xylanase genes. Constitutive overexpression ofcxrBin ∆gng-1circumvented the dependence of PBDE production on GNG-1. Further evidence indicated that CxrB indirectly regulated the transcription levels of key amylase genes by controlling the expression of the regulatory geneamyR. These data extended the diversity of Gγ protein functions and provided new insight into the signal transduction and regulation of PBDE gene expression in filamentous fungi.Key points•GNG-1 modulates the expression of PBDE genes and sporulation-related genes.•GNG-1 controls expression of the key regulatory gene cxrB.•Overexpression of cxrB circumvents dependence of PBDE production on GNG-1.