The tomato fer gene encoding a bHLH protein controls iron-uptake responses in roots
The tomato fer gene encoding a bHLH protein controls iron-uptake responses in roots
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DOI：
10.1073/pnas.212448699
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发表时间：
2002-10-15
影响因子：
11.1
通讯作者：
Ganal, M
中科院分区：
文献类型：
--
作者：
Ling, HQ;Bauer, P;Ganal, M
缺铁是植物中最常见的营养失调之一。为了科普低铁供应，除了禾本科植物增加溶解度和铁的吸收诱导生理和发育的变化，包括铁还原，土壤酸化，铁（II）运输和根毛增殖（策略I）。褪绿番茄fer突变体不能激活策略I。以前的研究表明，fer基因在根中是必需的。在这里，我们表明，FER植物表现出根发育表型后，低和足够的铁营养表明FER的行为，无论铁供应。突变体费尔根表现出较低的Leirt 1表达比野生型根。我们通过图位克隆的方法分离了fer基因，并证明它编码一个含有碱性螺旋-环-螺旋结构域的蛋白。FER在根尖以细胞特异性模式表达，与铁供应无关。我们的研究结果表明，FER可能控制根的生理和发育在转录水平上响应铁供应，因此可能是第一个确定的调节器在植物中的铁营养。
Iron deficiency is among the most common nutritional disorders in plants. To cope with low iron supply, plants with the exception of the Gramineae increase the solubility and uptake of iron by inducing physiological and developmental alterations including iron reduction, soil acidification, Fe(II) transport and root-hair proliferation (strategy I). The chlorotic tomato fer mutant fails to activate the strategy I. It was shown previously that the fer gene is required in the root. Here, we show that fer plants exhibit root developmental phenotypes after low and sufficient iron nutrition indicating that FER acts irrespective of iron supply. Mutant fer roots displayed lower Leirt1 expression than wild-type roots. We isolated the fer gene by map-based cloning and demonstrate that it encodes a protein containing a basic helix-loop-helix domain. fer is expressed in a cell-specific pattern at the root tip independently from iron supply. Our results suggest that FER may control root physiology and development at a transcriptional level in response to iron supply and thus may be the first identified regulator for iron nutrition in plants.