Loss of tumorigenicity with simultaneous changes in radiosensitivity and photosensitivity during in vitro growth of L5178Y murine lymphoma cells.
Loss of tumorigenicity with simultaneous changes in radiosensitivity and photosensitivity during in vitro growth of L5178Y murine lymphoma cells.
复制标题
L5178Y 鼠淋巴瘤细胞体外生长期间致瘤性丧失，同时放射敏感性和光敏感性发生变化。
作者：
J. Z. Beer;E. Budzicka;E. Niepokojczycka;O. Rosiek;I. Szumiel;M. Walicka
小鼠白血病淋巴母细胞L5178 Y-R（LY-R）在体外长时间（5个月至4年）培养后转化为L5178 Y-S（LY-S）变体。LY-R细胞在DBA/2小鼠中具有高度致瘤性;对X射线具有抗性[D 0（平均致死剂量[剂量-存活曲线线性部分斜率的倒数]）= 0.91格雷];对紫外线辐射（D 0 = 0.7 J/sq m）、短期（最多60 min）热（43度）治疗和某些潜在的抗癌药物敏感。LY-S细胞在DBA/2小鼠中几乎无致瘤性;对X射线敏感（D 0 = 0.56格雷）;对紫外线辐射（D 0 = 5.5 J/m2）、短期热处理和药物具有抗性。这两种细胞株对物理和化学试剂的敏感性的差异被DNA修复效率的差异所掩盖。虽然这两种菌株都可以在软琼脂中克隆，但LY-S细胞总是显示出更高的平板接种效率。LY-R和LY-S细胞的体外平均倍增时间分别为12至15小时和约10小时。与LY-R细胞转化为LY-S细胞相关的致瘤性的实际丧失以及放射敏感性和光敏性的变化在短时间内发生。这表明这些变化（以及可能的上述其他表型变化）是由体外培养条件引起的单个事件或在相对短的时间内发生的几个事件引起的。
Murine leukemic lymphoblasts L5178Y-R (LY-R) undergo conversion into their L5178Y-S (LY-S) variant as a result of prolonged (5 months to 4 years) cultivation in vitro. LY-R cells are highly tumorigenic in DBA/2 mice; resistant to X-rays [D0 (mean lethal dose [reciprocal of the slope of the linear portion of dose-survival curve] ) = 0.91 grays]; and sensitive to ultraviolet radiation (D0 = 0.7 J/sq m), short (up to 60 min) heat (43 degrees) treatment, and certain potential cancer drugs. LY-S cells are practically nontumorigenic in DBA/2 mice; sensitive to X-rays (D0 = 0.56 grays); and resistant to ultraviolet radiation (D0 = 5.5 J/sq m), short heat treatment, and the drugs. The differences in sensitivity of these two cell strains to physical and chemical agents are paralleled by differences in DNA repair efficiency. Although both strains can be cloned in soft agar, LY-S cells invariably show higher plating efficiencies. In vitro mean doubling times are 12 to 15 hr and approximately 10 hr for LY-R and LY-S cells, respectively. The actual loss of tumorigenicity and changes in radio- and photosensitivity associated with conversion of LY-R cells into LY-S cells occur within a short time. This indicates that these changes (and probably other phenotypic changes mentioned above) result from a single event or from several events occurring within a relatively short time elicited by in vitro culture conditions.
淋巴瘤细胞变体中的糖脂表达：化学量、免疫反应性以及与 NK 细胞易感性的相关性。
DOI：
--
发表时间：
1981
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
YoungJr,WW;Durdik,JM;Urdal,D;Hakomori,S;Henney,CS
通讯作者：
Henney,CS
X 照射的 L5178Y S/S 细胞中存活与 DNA 结构恢复的相关性。
影响因子：
3.4
作者：
Goldin,EM;Cox,AB;Lett,JT
通讯作者：
Lett,JT