An ubiquitin-like protein SDE2 negatively affects sucrose-induced anthocyanin biosynthesis in Arabidopsis
An ubiquitin-like protein SDE2 negatively affects sucrose-induced anthocyanin biosynthesis in Arabidopsis
复制标题
泛素样蛋白 SDE2 对拟南芥中蔗糖诱导的花青素生物合成产生负面影响
DOI：
10.1016/j.scib.2017.11.014
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发表时间：
2017
期刊：
影响因子：
18.9
通讯作者：
Jirong Huang
中科院分区：
文献类型：
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作者：
Jirong Huang
花色素苷的生物合成受一个由MYB、bHLH和WD 40亚基组成的保守的MBW转录复合体调控。然而，这些MBW亚基的转录调控的分子机制仍然很难理解。在这项研究中，我们分离出一个拟南芥突变体，显示。一个组成性的红色在地上组织生长迟缓的表型。在蔗糖存在下，突变体积累了野生型（WT）的3倍以上的花色素苷，但在不存在蔗糖的情况下不能产生WT的花色素苷。图位克隆结果表明，突变发生在At4G01000位点，该位点编码一个保守的核定位泛素样蛋白。(UBL)真核生物中的沉默缺陷2（SDE2）超家族蛋白。SDE2在各种组织中无处不在。expressed.in在蔗糖诱导的花色素苷合成中，SDE2的表达在前期不受蔗糖处理的影响，但在后期得到增强。responded.to SDE2突变导致花青素苷生物合成和调控基因的上调。酵母双杂交分析表明，SDE2与MYB转录因子PAP1和bHLH因子TT8没有直接的相互作用，说明SDE2是影响花青苷积累的间接因子。综上所述，我们的数据表明，SDE2可能在精细协调花青素苷生物合成与其他生物过程中发挥作用。
Anthocyanin biosynthesis is regulated by a conserved transcriptional MBW complex composed of MYB,.bHLH and WD40 subunits. However, molecular mechanisms underlying transcriptional regulation of.these MBW subunits remain largely elusive. In this study, we isolated an Arabidopsis mutant that displays.a constitutive red color in aboveground tissues with retarded growth phenotypes. In the presence of.sucrose, the mutant accumulates more than 3-fold anthocyanins of the wild type (WT), but cannot produce.anthocyanins as WT in the absence of sucrose. Map-based cloning results demonstrated that the.mutation occurs in the locus At4G01000, which encodes a conserved nuclear-localized ubiquitin-like.(UBL) superfamily protein, silencing defective 2 (SDE2), in eukaryotes. SDE2 is ubiquitously expressed.in various tissues. In the sucrose-induced anthocyanin biosynthesis, SDE2 expression was not responded.to sucrose treatment at the early stage but was enhanced at the late stage. SDE2 mutations result in upregulation.of anthocyanin biosynthetic and regulatory genes. Yeast-two hybrid analysis indicated that.SDE2 has no direct interaction with the MYB transcription factor PAP1 and bHLH factor TT8, indicating.that SDE2 is a indirect factor to affect anthocyanin accumulation. Taking together, our data suggest that.SDE2 may play a role in finely coordinating anthocyanin biosynthesis with other biological processes.