Acupuncture has a positive effect on asthmatic rats in a glucocorticoid-independent manner.
Acupuncture has a positive effect on asthmatic rats in a glucocorticoid-independent manner.
复制标题
针灸对哮喘大鼠具有不依赖糖皮质激素的积极作用
DOI：
10.1136/acupmed-2015-010934
复制
发表时间：
2016-12
期刊：
影响因子：
--
通讯作者：
Yang YQ
中科院分区：
文献类型：
--
作者：
Wang WQ;Xu YD;Cui LP;Yin LM;Wang Y;Liu YY;Yang YQ
有一些证据支持使用针灸作为哮喘的替代疗法。然而，其作用机制尚不完全清楚。虽然内源性糖皮质激素（GC）在调节免疫反应中起重要作用，但我们之前报道针灸对哮喘有有益作用，而不改变血清皮质醇的浓度。目的在哮喘模型诱导前对大鼠进行双侧肾上腺切除术（切除双肾上腺），探讨针刺是否以气相色谱依赖的方式影响哮喘。方法对肾上腺完整和肾上腺切除大鼠注射卵清蛋白诱导哮喘，然后不进行治疗或在GV14、双侧BL12和双侧BL13处进行手针刺（MA）治疗，或不进行手针刺。健康对照组和假肾上腺切除术对照组也包括在内。实验结束时测定肺阻力（RL）、血清皮质酮浓度和嗜酸性粒细胞计数。将经针刺治疗的肾上腺完整和去肾上腺哮喘大鼠血清注射到未治疗的肾上腺完整和去肾上腺哮喘大鼠体内，进一步探讨GC在针刺作用中的潜在作用。结果针刺可显著降低肾上腺未损伤大鼠和去肾上腺大鼠的RL和嗜酸性粒细胞计数。此外，针灸治疗的肾上腺完整和肾上腺切除的哮喘大鼠血清可以降低两种哮喘模型中RL和嗜酸性粒细胞计数的增加。结论本研究结果提示，内源性GC不是针灸治疗哮喘的关键因素，针灸可能以GC独立的方式对哮喘有潜在的治疗作用。
Background There is some evidence to support the use of acupuncture as an alternative therapy for asthma. However, the mechanisms underlying its effects are not fully understood. We have reported previously that acupuncture has beneficial effects on asthma without changing the concentration of serum cortisol, although endogenous glucocorticoid (GC) plays an important role in regulating immune responses. Objective In this study, bilateral adrenalectomy (removal of both adrenal glands) was performed in rats before asthma model induction to investigate whether acupuncture influences asthma in a GC-dependent manner. Methods Adrenal-intact and adrenalectomised rats were injected with ovalbumin to induce asthma and then left untreated or treated with manual acupuncture (MA) at GV14, bilateral BL12 and bilateral BL13, or manual restraint without MA. Healthy and sham-adrenalectomised control groups were also included. Pulmonary resistance (RL), serum concentrations of corticosterone, and eosinophil counts were measured at the end of the experimental course. Sera from adrenal-intact and adrenalectomised asthmatic rats treated with acupuncture were injected into untreated adrenal-intact and adrenalectomised asthmatic rats to investigate further the potential role of GC in the effect of acupuncture. Results Acupuncture significantly decreased RL and eosinophil count in both adrenal-intact and adrenalectomised asthmatic rats. Moreover, administration of sera derived from acupuncture-treated adrenal-intact and adrenalectomised asthmatic rats attenuated the increase in RL and eosinophil count in both asthmatic models. Conclusions Results of this study suggest that endogenous GC is not a key contributor to the effects of acupuncture on asthma, and that acupuncture may have potentially therapeutic effects on asthma in a GC-independent manner.