Isolation of rice genes possibly involved in the photoperiodic control of flowering by a fluorescent differential display method
Isolation of rice genes possibly involved in the photoperiodic control of flowering by a fluorescent differential display method
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DOI：
10.1093/pcp/pcf059
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发表时间：
2002-05-01
影响因子：
4.9
通讯作者：
Shimamoto, K
中科院分区：
文献类型：
--
作者：
Hayama, R;Izawa, T;Shimamoto, K
为了更好地理解水稻光周期调节的分子机制，我们通过使用荧光差异显示（FDD）从大约8，400个独立的mRNA物种中分离出27个在光周期不敏感se 5突变体中差异表达的cDNA。为了进行筛选，我们在夜间的五个不同时间点分离mRNA，并比较se 5和野生型之间的表达模式。在分离的27个cDNA中，有12个表现出与决定开花时间的基因相关的昼夜表达模式。在se 5中，9个cDNA-s的表达增加。其中5个cDNA在SD下表达上调，表明它们可能促进SD下的开花。它们包括编码含有推定NAC结构域、果糖二磷酸醛缩酶和蛋白酶抑制剂的cDNA的基因。在se 5中，三种cDNA的表达降低，但不受光周期调节。这些cDNA包括拟南芥GIGANTEA（GI）的水稻同源物，lir 1和肌醇1-磷酸合酶基因，所有这些基因先前都被证明是受生物钟控制的。水稻同源物的GI，OsGI，的表达模式相似的拟南芥GI，这表明这两个物种之间的光周期调节开花的一些机制的保护。
To better understand the molecular mechanisms of the photoperiodic regulation of rice, a short-day plant, we isolated 27 cDNAs that were differentially expressed in the photoperiod-insensitive se5 mutant from approximately 8,400 independent mRNA species by the use of a fluorescent differential display (FDD). For this screening, we isolated mRNAs at five different time points during the night and compared their expression patterns between se5 and the wild type. Of 27 cDNAs isolated, 12 showed diurnal expression patterns often associated with genes involved in the determination of the flowering time. In se5, expression of nine cDNA-s was increased. Five of these cDNAs were up-regulated under SD, suggesting that they may promote flowering under SD. They included genes encoding a cDNA containing a putative NAC domain, the fructose-bisphosphate aldolase, and a protease inhibitor. Expression of three cDNAs was decreased in se5 but not photoperiodically regulated. These cDNAs included a rice homolog of Arabidopsis GIGANTEA (GI), lir1, and a gene for myo-inositol 1-phosphate synthase, all of which were previously shown to be under the control of circadian clocks. The expression patterns of the rice homolog of GI, OsGI, were similar to those of the Arabidopsis GI, suggesting the conservation of some mechanisms for the photoperiodic regulation of flowering between these two species.