Gene expression under water deficit in loblolly pine (Pinus taeda): Isolation and characterization of cDNA clones
Gene expression under water deficit in loblolly pine (Pinus taeda): Isolation and characterization of cDNA clones
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DOI：
10.1111/j.1399-3054.1996.tb00490.x
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发表时间：
1996-05-01
影响因子：
6.4
通讯作者：
Cairney, J
中科院分区：
文献类型：
--
作者：
Chang, SJ;Puryear, JD;Cairney, J
从火炬松(Pinus taeda L.)根中提取聚A(+)RNA。苗木遭受逐渐和长期的水分亏缺。用此RNA构建了一个cDNA文库。分离到一批受水分胁迫诱导表达的基因克隆。进一步鉴定了其中4个克隆，分别命名为pLP2、pLP3、pLP4和pLP5。这些松树基因无论是在序列上还是在表达模式上都有独特的特征，pLP2编码的蛋白质与一系列植物的S腺苷蛋氨酸合成酶有91%的同源性。PLP3编码的蛋白与番茄在水分胁迫和果实成熟过程中诱导的蛋白相似，但松树蛋白在氨基端附近有一个独特的34个氨基酸区域。LP4蛋白与日本漆树中的铜结合蛋白Stellacyanin相似，但具有其他植物中没有的富含脯氨酸/丝氨酸的羧基末端。克隆pLP5编码一种新的富含甘氨酸的蛋白，与丝素蛋白和大鼠软骨素核心蛋白相似，但推测的松树蛋白不同于先前描述的富含甘氨酸的蛋白。除pLP5基因外，4个基因的转录水平在中度水分胁迫下升高，然后随着胁迫的加重(缺水1个月)而下降，即使在重度胁迫下也保持在较高水平。对编码蛋白在细胞壁强化中的可能作用进行了讨论。
Poly(A(+)) RNA was extracted from roots of loblolly pine (Pinus taeda L.) seedlings subjected to gradual and prolonged water deficit. This RNA was used to construct a cDNA library. A number of cDNA clones were isolated whose expression was induced by water deficit. Four of these cDNA clones, designated pLP2, pLP3, pLP4 and pLP5, were characterized further. Each of these pine genes has unique characteristics either in sequence or pattern of expression, The protein encoded by pLP2 shows 91% identity to S-adenosylmethionine synthetase from a range of plants. The protein encoded by pLP3 is similar to a tomato protein induced by water deficit and during fruit ripening, but the pine protein possesses a unique 34-amino-acid region near the amino terminal. The LP4 protein is similar to stellacyanin, a copper-binding protein in the Japanese lacquer tree, but possesses a proline/serine-rich carboxy terminus not found in other plants. Clone pLP5 encodes a novel glycine-rich protein with similarity to both silk fibroin and the rat chondroitin core protein, but the putative pine protein is distinct from previously characterized glycine-rich proteins. Transcript levels of the four genes rose under moderate water deficit stress and then declined as stress became severe (1 month without water), with the exception of pLP5 mRNA, which remained at elevated levels even under severe stress. The possible roles of the encoded proteins in cell wall reinforcement are discussed.