Identification of two alpha-subunit species of GTP-binding proteins, Galpha15 and Galphaq, expressed in rat taste buds.
Identification of two alpha-subunit species of GTP-binding proteins, Galpha15 and Galphaq, expressed in rat taste buds.
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鉴定大鼠味蕾中表达的 GTP 结合蛋白 Galpha15 和 Galphaq 的两种 α 亚基。
DOI：
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发表时间：
1998
期刊：
影响因子：
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通讯作者：
K. Abe
中科院分区：
文献类型：
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作者：
Y. Kusakabe;E. Yamaguchi;K. Tanemura;K. Kameyama;N. Chiba;S. Arai;Y. Emori;K. Abe
我们从大鼠舌头中克隆了属于 Galphaq 家族的两个 G 蛋白 α 亚基的 cDNA，每个亚基都能够激活 PLCbeta。一种是狭义上的Galphaq，另一种称为大鼠Galpha15，是小鼠Galpha15的大鼠对应物，氨基酸序列相似性为94%。 RT-PCR和Northern印迹分析表明，大鼠Galpha15和Galphaq在含有味蕾的舌上皮中明显表达。免疫染色还表明，大鼠 Galpha15 与 Galphaq 主要位于味蕾中。这些研究表明这两种 Galpha 蛋白可能在感觉细胞中发挥味觉信号转导的作用。
We cloned cDNAs for two G protein alpha-subunits belonging to the Galphaq family, each capable of activating PLCbeta, from rat tongue. One is a Galphaq in the narrow sense, and the other, termed rat Galpha15, is a rat counterpart of mouse Galpha15, sharing an amino acid sequence similarity of 94%. RT-PCR and Northern blot analysis demonstrated that rat Galpha15 and Galphaq were distinctly expressed in tongue epithelia containing taste buds. Immunostaining also showed that rat Galpha15, together with the Galphaq, was localized mainly in taste buds. These studies suggest the possibility that these two Galpha proteins function for taste signal transduction in sensory cells.