Formation of a FGF-2 and calcium phosphate composite layer on a hydroxyapatite ceramic for promoting bone formation
Formation of a FGF-2 and calcium phosphate composite layer on a hydroxyapatite ceramic for promoting bone formation
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DOI：
10.1088/1748-6041/2/3/s16
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发表时间：
2007-09-01
影响因子：
4
通讯作者：
Ichinose, Noboru
中科院分区：
文献类型：
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作者：
Sogo, Yu;Ito, Atsuo;Ichinose, Noboru
将成纤维细胞生长因子-2固定在羟基磷灰石(HAP)陶瓷上，用临床批准的输液配制成过饱和的磷酸钙溶液。为了避免成纤维细胞生长因子-2变性的风险，在25℃下进行了成纤维细胞生长因子-2的固定化，用磷酸钙沉积的方法成功地将成纤维细胞生长因子-2固定在羟基磷灰石陶瓷表面，形成了成纤维细胞生长因子-2和磷酸钙复合层。在最佳条件下，在HAP陶瓷表面形成的复合层中可固定化最大为2.72+/-0.01微克厘米(-2)的成纤维细胞生长因子-2。固定成纤维细胞生长因子-2的羟基磷灰石陶瓷可能具有释放足够量的成纤维细胞生长因子-2以促进骨形成的能力。由于成纤维细胞NIH3T3的增殖被促进，因此从固定成纤维细胞的羟基磷灰石陶瓷中释放出的成纤维细胞生长因子-2保持了其生物学活性。因此，将成纤维细胞生长因子-2固定的羟基磷灰石陶瓷有望成为促进新骨形成的有用材料。
Fibroblast growth factor-2 (FGF-2) was immobilized on a hydroxyapatite (HAP) ceramic in supersaturated calcium phosphate solution prepared using solutions corresponding to clinically approved infusion fluids. To avoid the risk of FGF-2 denaturation, FGF-2 immobilization was carried out at 25 degrees C. FGF-2 was successfully immobilized on HAP ceramic surfaces by deposition with calcium phosphate to form a FGF-2 and calcium phosphate composite layer. A maximum of 2.72 +/- 0.01 mu g cm(-2) of FGF-2 was immobilized in the composite layer formed on the HAP ceramic under the optimum condition. A FGF-2-immobilized HAP ceramic is likely to have the ability to release a sufficient amount of FGF-2 to promote bone formation. FGF-2 released from a FGF-2-immobilized HAP ceramic maintained its biological activity, since the proliferation of fibroblastic NIH3T3 was promoted. Therefore, the FGF-2-immobilized HAP ceramic is expected to be a useful material for promoting new bone formation.